Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/91401
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Title: Varifocal metalens for optical sectioning fluorescence microscopy
Authors: Luo, Y
Chu, CH
Vyas, S
Kuo, HY
Chia, YH
Chen, MK 
Shi, X
Tanaka, T
Misawa, H
Huang, YY
Tsai, DP 
Issue Date: Jun-2021
Source: Nano letters, 23 June 2021, v. 21, no. 12, p. 5133-5142
Abstract: Fluorescence microscopy with optical sectioning capabilities is extensively utilized in biological research to obtain three-dimensional structural images of volumetric samples. Tunable lenses have been applied in microscopy for axial scanning to acquire multiplane images. However, images acquired by conventional tunable lenses suffer from spherical aberration and distortions. Here, we design, fabricate, and implement a dielectric Moiré metalens for fluorescence imaging. The Moiré metalens consists of two complementary phase metasurfaces, with variable focal length, ranging from ∼10 to ∼125 mm at 532 nm by tuning mutual angles. In addition, a telecentric configuration using the Moiré metalens is designed for high-contrast multiplane fluorescence imaging. The performance of our system is evaluated by optically sectioned images obtained from HiLo illumination of fluorescently labeled beads, as well as ex vivo mice intestine tissue samples. The compact design of the varifocal metalens may find important applications in fluorescence microscopy and endoscopy for clinical purposes.
Keywords: Fluorescence microscopy
HiLo imaging
Metasurface
Optical sectioning
Telecentric configuration
Publisher: American Chemical Society
Journal: Nano letters 
ISSN: 1530-6984
EISSN: 1530-6992
DOI: 10.1021/acs.nanolett.1c01114
Rights: © 2021 The Authors. Published by American Chemical Society
This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/)
The following publication Luo, Y., Chu, C. H., Vyas, S., Kuo, H. Y., Chia, Y. H., Chen, M. K., ... & Tsai, D. P. (2021). Varifocal Metalens for Optical Sectioning Fluorescence Microscopy. Nano Letters is available at https://doi.org/10.1021/acs.nanolett.1c01114
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