Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/91054
| Title: | Combined retinal proteome datasets in response to atropine treatment using iTRAQ and SWATH-MS based proteomics approaches in guinea pig myopia model | Authors: | Zhu, Y Bian, JF Lu, DQ Wang, Q Gong, BT Li, KK Yu, FJ Cheung, JKW Ji, XW Zhang, HM Du, B Nian, H To, CH Wei, RH Lam, TC |
Issue Date: | Dec-2020 | Source: | Data in brief, Dec. 2020, v. 33, 106526 | Abstract: | Atropine, a non-selective muscarinic antagonist, is known to slow down myopia progression in human adolescents and in several animal models. However, its underlying molecular mechanism is unclear. The present work built a monocular form-deprivation myopia (FDM) guinea pig model, using facemasks as well as atropine treatment on FDM eyes for 2 and 4 weeks. Retinal protein changes in response to the FDM and effects of topical administration of atropine were screened for the two periods using fractionated isobaric tags for a relative and absolute quantification (iTRAQ) approach coupled with nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) (n=24, 48 eyes). Retinal tissues from another cohort receiving 4-weeks FDM with atropine treatment (n=12, 24 eyes) with more significant changes were subjected to sequential window acquisition of all theoretical mass spectra (SWATH-MS) proteomics for further protein target confirmation. A total of 1695 pro-teins (8875 peptides) and 5961 proteins (51871 peptides) were identified using iTRAQ and SWATH approaches, respectively. Using the Paragon algorithm in the ProteinPilot (TM) software, the three most significantly up-regulated and down-regulated proteins that were commonly found in both ITRAQ and SWATH experiments are presented. All raw data generated from the work were submitted and published in the Peptide Atlas public repository (http://www.peptideatlas.org/) for general release (Data ID PASS01507). | Keywords: | Guinea pigs FDM Atropine ITRAQ SWATH |
Publisher: | Elsevier | Journal: | Data in brief | ISSN: | 2352-3409 | DOI: | 10.1016/j.dib.2020.106526 | Rights: | © 2020 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) The following publication Ying Zhu, Jingfang Bian, Daqian Lu, Qiong Wang, Boteng Gong, King-Kit Li, Fengjuan Yu, Jimmy Ka-Wai Cheung, Xiaowen Ji, Hongmei Zhang, Bei Du, Hong Nian, Chi-ho To, Ruihua Wei, Thomas Chuen Lam, Combined retinal proteome datasets in response to atropine treatment using iTRAQ and SWATH-MS based proteomics approaches in guinea pig myopia model, Data in Brief, Volume 33, 2020, 106526, ISSN 2352-3409, https://doi.org/10.1016/j.dib.2020.106526 is available at (https://www.sciencedirect.com/science/article/pii/S2352340920314086) |
| Appears in Collections: | Journal/Magazine Article |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Bian_Combined_retinal_proteome.pdf | 1.49 MB | Adobe PDF | View/Open |
Access
View full-text via PolyU eLinks 
Page views
17
Last Week
1
1
Last month
Citations as of Jun 4, 2023
Downloads
2
Citations as of Jun 4, 2023
SCOPUSTM
Citations
3
Citations as of Jun 8, 2023
WEB OF SCIENCETM
Citations
2
Citations as of Jun 8, 2023
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.