Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/91054
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dc.contributorSchool of Optometryen_US
dc.creatorZhu, Yen_US
dc.creatorBian, JFen_US
dc.creatorLu, DQen_US
dc.creatorWang, Qen_US
dc.creatorGong, BTen_US
dc.creatorLi, KKen_US
dc.creatorYu, FJen_US
dc.creatorCheung, JKWen_US
dc.creatorJi, XWen_US
dc.creatorZhang, HMen_US
dc.creatorDu, Ben_US
dc.creatorNian, Hen_US
dc.creatorTo, CHen_US
dc.creatorWei, RHen_US
dc.creatorLam, TCen_US
dc.date.accessioned2021-09-09T03:39:17Z-
dc.date.available2021-09-09T03:39:17Z-
dc.identifier.issn2352-3409en_US
dc.identifier.urihttp://hdl.handle.net/10397/91054-
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2020 The Authors. Published by Elsevier Inc.en_US
dc.rightsThis is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)en_US
dc.rightsThe following publication Ying Zhu, Jingfang Bian, Daqian Lu, Qiong Wang, Boteng Gong, King-Kit Li, Fengjuan Yu, Jimmy Ka-Wai Cheung, Xiaowen Ji, Hongmei Zhang, Bei Du, Hong Nian, Chi-ho To, Ruihua Wei, Thomas Chuen Lam, Combined retinal proteome datasets in response to atropine treatment using iTRAQ and SWATH-MS based proteomics approaches in guinea pig myopia model, Data in Brief, Volume 33, 2020, 106526, ISSN 2352-3409 is available at https://doi.org/10.1016/j.dib.2020.106526.en_US
dc.subjectGuinea pigsen_US
dc.subjectFDMen_US
dc.subjectAtropineen_US
dc.subjectITRAQen_US
dc.subjectSWATHen_US
dc.titleCombined retinal proteome datasets in response to atropine treatment using iTRAQ and SWATH-MS based proteomics approaches in guinea pig myopia modelen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.volume33en_US
dc.identifier.doi10.1016/j.dib.2020.106526en_US
dcterms.abstractAtropine, a non-selective muscarinic antagonist, is known to slow down myopia progression in human adolescents and in several animal models. However, its underlying molecular mechanism is unclear. The present work built a monocular form-deprivation myopia (FDM) guinea pig model, using facemasks as well as atropine treatment on FDM eyes for 2 and 4 weeks. Retinal protein changes in response to the FDM and effects of topical administration of atropine were screened for the two periods using fractionated isobaric tags for a relative and absolute quantification (iTRAQ) approach coupled with nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) (n=24, 48 eyes). Retinal tissues from another cohort receiving 4-weeks FDM with atropine treatment (n=12, 24 eyes) with more significant changes were subjected to sequential window acquisition of all theoretical mass spectra (SWATH-MS) proteomics for further protein target confirmation. A total of 1695 pro-teins (8875 peptides) and 5961 proteins (51871 peptides) were identified using iTRAQ and SWATH approaches, respectively. Using the Paragon algorithm in the ProteinPilot (TM) software, the three most significantly up-regulated and down-regulated proteins that were commonly found in both ITRAQ and SWATH experiments are presented. All raw data generated from the work were submitted and published in the Peptide Atlas public repository (http://www.peptideatlas.org/) for general release (Data ID PASS01507).en_US
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationData in brief, Dec. 2020, v. 33, 106526en_US
dcterms.isPartOfData in briefen_US
dcterms.issued2020-12-
dc.identifier.isiWOS:000600652300191-
dc.identifier.pmid33304948-
dc.identifier.artn106526en_US
dc.description.validate202109 bchyen_US
dc.description.oaVersion of Recorden_US
dc.identifier.FolderNumberOA_Scopus/WOS-
dc.description.pubStatusPublisheden_US
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