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Title: Rational design of Red fluorescent and selective G-quadruplex DNA sensing probes : the study of interaction signaling and the molecular structural relationship achieving high specificity
Authors: Zheng, BX
Long, W
Zhang, YH
Huang, XH
Chen, CC
Zhong, DX
She, MT
Chen, ZX
Cai, DP
Lu, YJ
Wong, WL 
Issue Date: 1-Jul-2020
Source: Sensors and actuators. B, Chemical, 1 July 2020, v. 314, 128075, p. 1-12
Abstract: Red fluorescent G-quadruplex DNA selective organic binding probe is relatively underdeveloped. In the present study, we designed a series of new fluorescent probes that exhibit large Stokes shift (112−131 nm), high quantum yield (up to 0.56), robust against photo-bleaching and high selectivity towards quadruplex structures of c-MYC and telomere DNA with strong binding affinity. The discrimination ability of the probes towards G-quadruplex structures against single-/double-stranded DNA or RNA was investigated by analyzing their structural property with respect to their binding specificity. From the sensing application of the probe targeting pu27, an excellent linear relationship (R2 = 0.9997) with respect to the pu27-A3 interaction signal was observed. The LOD was found down to 4.0 nM, which is superior to thiazole orange dye. The probe is able to realize real-time fluorescence visualization in aqueous, PAGE staining and live cell imaging. In addition, from the immunofluorescence analysis using the probe and BG4 antibody, the A3 foci and BG4 staining co-localized well in PC3 cells, which suggest that the probe is G-quadruplex selective and is useful for visualizing G-quadruplex DNA structures in live cells. Cytotoxicity assays also demonstrated that the probe has low cytotoxicity against both PC3 cancer cells and 16HBE normal cells.
Keywords: BG4 antibody co-localization
c-myc DNA recognition
DNA structure discrimination
Live cell imaging
Red fluorescent quadruplex DNA probes
Publisher: Elsevier
Journal: Sensors and actuators. B, Chemical 
ISSN: 0925-4005
EISSN: 1873-3077
DOI: 10.1016/j.snb.2020.128075
Rights: © 2020 Elsevier B.V. All rights reserved.
© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
The following publication Zheng, B.-X., Long, W., Zhang, Y.-H., Huang, X.-H., Chen, C.-C., Zhong, D.-X., She, M.-T., Chen, Z.-X., Cai, D.-P., Lu, Y.-J., & Wong, W.-L. (2020). Rational design of Red fluorescent and selective G-quadruplex DNA sensing probes: The study of interaction signaling and the molecular structural relationship achieving high specificity. Sensors and Actuators B: Chemical, 314, 128075 is available at
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