Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/88440
PIRA download icon_1.1View/Download Full Text
DC FieldValueLanguage
dc.contributorDepartment of Applied Biology and Chemical Technologyen_US
dc.creatorZheng, BXen_US
dc.creatorLong, Wen_US
dc.creatorZhang, YHen_US
dc.creatorHuang, XHen_US
dc.creatorChen, CCen_US
dc.creatorZhong, DXen_US
dc.creatorShe, MTen_US
dc.creatorChen, ZXen_US
dc.creatorCai, DPen_US
dc.creatorLu, YJen_US
dc.creatorWong, WLen_US
dc.date.accessioned2020-11-19T03:51:44Z-
dc.date.available2020-11-19T03:51:44Z-
dc.identifier.issn0925-4005en_US
dc.identifier.urihttp://hdl.handle.net/10397/88440-
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2020 Elsevier B.V. All rights reserved.en_US
dc.rights© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.en_US
dc.rightsThe following publication Zheng, B.-X., Long, W., Zhang, Y.-H., Huang, X.-H., Chen, C.-C., Zhong, D.-X., She, M.-T., Chen, Z.-X., Cai, D.-P., Lu, Y.-J., & Wong, W.-L. (2020). Rational design of Red fluorescent and selective G-quadruplex DNA sensing probes: The study of interaction signaling and the molecular structural relationship achieving high specificity. Sensors and Actuators B: Chemical, 314, 128075 is available at https://dx.doi.org/10.1016/j.snb.2020.128075.en_US
dc.subjectBG4 antibody co-localizationen_US
dc.subjectc-myc DNA recognitionen_US
dc.subjectDNA structure discriminationen_US
dc.subjectLive cell imagingen_US
dc.subjectRed fluorescent quadruplex DNA probesen_US
dc.titleRational design of Red fluorescent and selective G-quadruplex DNA sensing probes : the study of interaction signaling and the molecular structural relationship achieving high specificityen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage1en_US
dc.identifier.epage12en_US
dc.identifier.volume314en_US
dc.identifier.doi10.1016/j.snb.2020.128075en_US
dcterms.abstractRed fluorescent G-quadruplex DNA selective organic binding probe is relatively underdeveloped. In the present study, we designed a series of new fluorescent probes that exhibit large Stokes shift (112−131 nm), high quantum yield (up to 0.56), robust against photo-bleaching and high selectivity towards quadruplex structures of c-MYC and telomere DNA with strong binding affinity. The discrimination ability of the probes towards G-quadruplex structures against single-/double-stranded DNA or RNA was investigated by analyzing their structural property with respect to their binding specificity. From the sensing application of the probe targeting pu27, an excellent linear relationship (R2 = 0.9997) with respect to the pu27-A3 interaction signal was observed. The LOD was found down to 4.0 nM, which is superior to thiazole orange dye. The probe is able to realize real-time fluorescence visualization in aqueous, PAGE staining and live cell imaging. In addition, from the immunofluorescence analysis using the probe and BG4 antibody, the A3 foci and BG4 staining co-localized well in PC3 cells, which suggest that the probe is G-quadruplex selective and is useful for visualizing G-quadruplex DNA structures in live cells. Cytotoxicity assays also demonstrated that the probe has low cytotoxicity against both PC3 cancer cells and 16HBE normal cells.en_US
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationSensors and actuators. B, Chemical, 1 July 2020, v. 314, 128075, p. 1-12en_US
dcterms.isPartOfSensors and actuators. B, Chemicalen_US
dcterms.issued2020-07-01-
dc.identifier.scopus2-s2.0-85083366043-
dc.identifier.eissn1873-3077en_US
dc.identifier.artn128075en_US
dc.description.validate202011 bcrcen_US
dc.description.oaAccepted Manuscripten_US
dc.identifier.FolderNumbera0505-n02-
dc.description.pubStatusPublisheden_US
Appears in Collections:Journal/Magazine Article
Files in This Item:
File Description SizeFormat 
Zheng_Rational_Red_Fluorescent.pdfPre-Published version5.52 MBAdobe PDFView/Open
Open Access Information
Status open access
File Version Final Accepted Manuscript
Access
View full-text via PolyU eLinks SFX Query
Show simple item record

Page views

112
Last Week
0
Last month
Citations as of Mar 24, 2024

Downloads

18
Citations as of Mar 24, 2024

SCOPUSTM   
Citations

13
Citations as of Mar 28, 2024

WEB OF SCIENCETM
Citations

13
Citations as of Mar 28, 2024

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.