Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/88306
PIRA download icon_1.1View/Download Full Text
Title: Vanillin inhibits PqsR-mediated virulence in Pseudomonas aeruginosa
Authors: Mok, N
Chan, SY 
Liu, SY 
Chua, SL 
Issue Date: 1-Jul-2020
Source: Food and function, 1 July 2020, v. 11, p. 6496-6508
Abstract: Reduced efficacy of antibiotics in bacterial diseases is a global concern in clinical settings. Development of anti-virulence compounds which disarm bacterial virulence is an attractive therapeutic agent for complementary antibiotics usage. One potential target for anti-virulence compounds is quorum sensing (QS), the intercellular communication system in most pathogens, such as Pseudomonas aeruginosa. QS inhibitors (QSIs) can inhibit QS effectively, attenuate QS-mediated virulence, and improve host clearance of infections. While studies focused on developing homoserine-based las QSI, few targeted the quinolone-based pqs QS, which implicated host cytotoxicity and biofilm formation. It is imperative to develop novel anti-pqs-QS therapeutics for combinatorial antibiotic treatment of microbial diseases. We employed a gfp-based transcriptional pqs biosensor to screen a natural compounds library and identify vanillin (4-hydroxy-3-methoxybenzaldehyde), the primary phenolic aldehyde of vanilla bean. The vanillin inhibited pqs expression and its associated phenotypes, namely pyocyanin production and twitching motility in P. aeruginosa. Molecular docking results revealed that vanillin binds to the active site of PqsR, the PQS-binding response regulator. Combinatorial treatment of vanillin with antimicrobial peptide (colistin) inhibited biofilm growth in vitro and improved treatment in the in vivo C. elegans acute infection model. We demonstrated that vanillin could dampen pqs QS and associated virulence, thus providing novel therapeutic strategies against P. aeruginosa infections.
Publisher: Royal Society of Chemistry
Journal: Food and function 
ISSN: 2042-6496
EISSN: 2042-650X
DOI: 10.1039/d0fo00046a
Rights: This journal is © The Royal Society of Chemistry 2020
Appears in Collections:Journal/Magazine Article

Files in This Item:
File Description SizeFormat 
Mok_Vanillin_Inhibits_PqsR-mediated.pdfPre-Published version2.15 MBAdobe PDFView/Open
Open Access Information
Status open access
File Version Final Accepted Manuscript
Access
View full-text via PolyU eLinks SFX Query
Show full item record

Page views

38
Citations as of Jul 3, 2022

Downloads

25
Citations as of Jul 3, 2022

SCOPUSTM   
Citations

12
Citations as of Jul 7, 2022

WEB OF SCIENCETM
Citations

11
Citations as of Jul 7, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.