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dc.contributorDepartment of Applied Biology and Chemical Technologyen_US
dc.creatorMok, Nen_US
dc.creatorChan, SYen_US
dc.creatorLiu, SYen_US
dc.creatorChua, SLen_US
dc.date.accessioned2020-10-27T04:40:47Z-
dc.date.available2020-10-27T04:40:47Z-
dc.identifier.issn2042-6496en_US
dc.identifier.urihttp://hdl.handle.net/10397/88306-
dc.language.isoenen_US
dc.publisherRoyal Society of Chemistryen_US
dc.rightsThis journal is © The Royal Society of Chemistry 2020en US
dc.titleVanillin inhibits PqsR-mediated virulence in Pseudomonas aeruginosaen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage6496en_US
dc.identifier.epage6508en_US
dc.identifier.volume11en_US
dc.identifier.doi10.1039/d0fo00046aen_US
dcterms.abstractReduced efficacy of antibiotics in bacterial diseases is a global concern in clinical settings. Development of anti-virulence compounds which disarm bacterial virulence is an attractive therapeutic agent for complementary antibiotics usage. One potential target for anti-virulence compounds is quorum sensing (QS), the intercellular communication system in most pathogens, such as Pseudomonas aeruginosa. QS inhibitors (QSIs) can inhibit QS effectively, attenuate QS-mediated virulence, and improve host clearance of infections. While studies focused on developing homoserine-based las QSI, few targeted the quinolone-based pqs QS, which implicated host cytotoxicity and biofilm formation. It is imperative to develop novel anti-pqs-QS therapeutics for combinatorial antibiotic treatment of microbial diseases. We employed a gfp-based transcriptional pqs biosensor to screen a natural compounds library and identify vanillin (4-hydroxy-3-methoxybenzaldehyde), the primary phenolic aldehyde of vanilla bean. The vanillin inhibited pqs expression and its associated phenotypes, namely pyocyanin production and twitching motility in P. aeruginosa. Molecular docking results revealed that vanillin binds to the active site of PqsR, the PQS-binding response regulator. Combinatorial treatment of vanillin with antimicrobial peptide (colistin) inhibited biofilm growth in vitro and improved treatment in the in vivo C. elegans acute infection model. We demonstrated that vanillin could dampen pqs QS and associated virulence, thus providing novel therapeutic strategies against P. aeruginosa infections.en_US
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationFood and function, 1 July 2020, v. 11, p. 6496-6508en_US
dcterms.isPartOfFood and functionen_US
dcterms.issued2020-07-01-
dc.identifier.eissn2042-650Xen_US
dc.description.validate202010 bcrcen_US
dc.description.oaAccepted Manuscripten_US
dc.identifier.FolderNumbera0491-n04-
dc.description.pubStatusPublisheden_US
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