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Title: A microfluidic flow-through chip integrated with reduced graphene oxide transistor for influenza virus gene detection
Authors: Chan, C 
Shi, J 
Fan, Y 
Yang, M 
Issue Date: Nov-2017
Source: Sensors and actuators. B, Chemical, Nov. 2017, v. 251, p. 927-933
Abstract: Most of the current graphene transistor based deoxyribonucleic acid (DNA) sensors are based on dip and-dry methods The flow-through approach for graphene transistor based DNA sensors have not been explored yet. Moreover, the effect of probe immobilization strategies on the performance of a graphene transistor biosensor in flowing environment was rarely studied. In this paper, a microfluidic integrated reduced graphene oxide (rGO) transistor was developed for H5N1 influenza virus gene detection with high stability and sensitivity via a flow-through strategy. Different DNA probe immobilization approaches including extended long capture probe via Tr-Tr stacking, short capture probe via Tr-Tr stacking and covalent immobilization via linker were studied. Both fluorescence measurement and electrical detection were performed to evaluate the performance of rGO transistors in flowing environment for these probe immobilization strategies. The results showed that among these approaches, extended long capture probe could provide both high sensitivity and stability in flowing environment while short capture probe suffered by the low stability in flowing environment and covalent immobilization via linker had relatively low sensitivity. This microfluidic integrated rGO transistor with extended capture probe immobilization approach could provide a promising platform for nucleic acid detection with high sensitivity and stability for potential flow-through chip application.
Keywords: Microfluidic device
Flow-through chip
Reduced graphene oxide (rGO)
Publisher: Elsevier
Journal: Sensors and actuators. B, Chemical 
ISSN: 0925-4005
EISSN: 1873-3077
DOI: 10.1016/j.snb.2017.05.147
Rights: © 2017 Elsevier B.V. All rights reserved.
© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
The following publication Chan, C., Shi, J., Fan, Y., & Yang, M. (2017). A microfluidic flow-through chip integrated with reduced graphene oxide transistor for influenza virus gene detection. Sensors and Actuators B: Chemical, 251, 927-933. is available at
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