Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/75724
PIRA download icon_1.1View/Download Full Text
DC FieldValueLanguage
dc.contributorDepartment of Biomedical Engineeringen_US
dc.creatorChan, Cen_US
dc.creatorShi, Jen_US
dc.creatorFan, Yen_US
dc.creatorYang, Men_US
dc.date.accessioned2018-05-10T02:54:28Z-
dc.date.available2018-05-10T02:54:28Z-
dc.identifier.issn0925-4005en_US
dc.identifier.urihttp://hdl.handle.net/10397/75724-
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2017 Elsevier B.V. All rights reserved.en_US
dc.rights© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.rightsThe following publication Chan, C., Shi, J., Fan, Y., & Yang, M. (2017). A microfluidic flow-through chip integrated with reduced graphene oxide transistor for influenza virus gene detection. Sensors and Actuators B: Chemical, 251, 927-933. is available at https://doi.org/10.1016/j.snb.2017.05.147en_US
dc.subjectMicrofluidic deviceen_US
dc.subjectFlow-through chipen_US
dc.subjectReduced graphene oxide (rGO)en_US
dc.subjectTransistoren_US
dc.titleA microfluidic flow-through chip integrated with reduced graphene oxide transistor for influenza virus gene detectionen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage927en_US
dc.identifier.epage933en_US
dc.identifier.volume251en_US
dc.identifier.doi10.1016/j.snb.2017.05.147en_US
dcterms.abstractMost of the current graphene transistor based deoxyribonucleic acid (DNA) sensors are based on dip and-dry methods The flow-through approach for graphene transistor based DNA sensors have not been explored yet. Moreover, the effect of probe immobilization strategies on the performance of a graphene transistor biosensor in flowing environment was rarely studied. In this paper, a microfluidic integrated reduced graphene oxide (rGO) transistor was developed for H5N1 influenza virus gene detection with high stability and sensitivity via a flow-through strategy. Different DNA probe immobilization approaches including extended long capture probe via Tr-Tr stacking, short capture probe via Tr-Tr stacking and covalent immobilization via linker were studied. Both fluorescence measurement and electrical detection were performed to evaluate the performance of rGO transistors in flowing environment for these probe immobilization strategies. The results showed that among these approaches, extended long capture probe could provide both high sensitivity and stability in flowing environment while short capture probe suffered by the low stability in flowing environment and covalent immobilization via linker had relatively low sensitivity. This microfluidic integrated rGO transistor with extended capture probe immobilization approach could provide a promising platform for nucleic acid detection with high sensitivity and stability for potential flow-through chip application.en_US
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationSensors and actuators. B, Chemical, Nov. 2017, v. 251, p. 927-933en_US
dcterms.isPartOfSensors and actuators. B, Chemicalen_US
dcterms.issued2017-11-
dc.identifier.isiWOS:000406184600112-
dc.identifier.eissn1873-3077en_US
dc.description.validate201805 bcrcen_US
dc.description.oaAccepted Manuscripten_US
dc.identifier.FolderNumberBME-0206-
dc.description.fundingSourceRGCen_US
dc.description.fundingSourceOthersen_US
dc.description.fundingTextHong Kong Research Granst Council; Hong Kong Polytechnic Universityen_US
dc.description.pubStatusPublisheden_US
dc.identifier.OPUS6750086-
Appears in Collections:Journal/Magazine Article
Files in This Item:
File Description SizeFormat 
Fan_Microfluidic_Flow-Through_Chip.pdfPre-Published version1 MBAdobe PDFView/Open
Open Access Information
Status open access
File Version Final Accepted Manuscript
Access
View full-text via PolyU eLinks SFX Query
Show simple item record

Page views

122
Last Week
0
Last month
Citations as of Oct 1, 2023

Downloads

43
Citations as of Oct 1, 2023

SCOPUSTM   
Citations

58
Last Week
0
Last month
Citations as of Sep 28, 2023

WEB OF SCIENCETM
Citations

46
Last Week
0
Last month
Citations as of Sep 28, 2023

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.