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Title: Non-invasive potential circulating mRNA markers for colorectal adenoma using targeted sequencing
Authors: Xue, VW 
Cheung, MT
Chan, PT
Luk, LLY
Lee, VH
Au, TC
Yu, AC
Cho, WCS
Tsang, HFA 
Chan, AK
Wong, SCC 
Issue Date: 2019
Source: Scientific reports, 10 Sept. 2019, v. 9, 12943, p. 1-10
Abstract: We have developed an optimized protocol for plasma targeted mRNA sequencing in our previous study. Here, we performed plasma targeted mRNA sequencing for 40 colorectal adenoma patients and 39 colonoscopy-proven normal controls in order to find potential circulating mRNA markers for colorectal adenoma. Results showed that GSK3A and RHOA were differential expressed genes identified by a cut-off of fold change >2 and adjusted P value < 0.05. More detailed analysis showed that the expression of both GSK3A (0.01-fold with adjusted P < 1 x 10(-6)) and RHOA (0.35-fold with adjusted P < 0.01) in adenoma patients was significantly lower than those in normal healthy subjects. Based on the enrichment analysis of biological process for potential markers, we found that the regulation of programmed cell death (GO: 0043067; GO: 0043069), regulation of cell death (GO: 0010941; GO: 0060548) and cell differentiation (GO: 0021861) were the main processes involved in adenoma formation. In summary, this study is a cutting-edge research on the detection of plasma mRNA in colorectal adenoma patients and normal healthy subjects.
Publisher: Nature Publishing Group
Journal: Scientific reports 
EISSN: 2045-2322
DOI: 10.1038/s41598-019-49445-x
Rights: Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre-ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per-mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
© The Author(s) 2019
The following publication Xue, V.W., Cheung, M.T., Chan, P.T. et al. Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing. Sci Rep 9, 12943 (2019), 1-10 is available at https://dx.doi.org/10.1038/s41598-019-49445-x
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