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Title: Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors
Authors: Zhu, Y 
Huang, ZY
Chen, Q 
Wu, Q 
Huang, XW
So, PK
Shao, LY
Yao, Z 
Jia, YW
Li, ZH
Yu, WX
Yang, Y
Jian, AQ
Sang, SB
Zhang, WD
Zhang, XM 
Issue Date: 2019
Source: Nature communications, 6 Sept. 2019, v. 10, 4049, p. 1-9
Abstract: Food production in green crops is severely limited by low activity and poor specificity of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) in natural photosynthesis (NPS). This work presents a scientific solution to overcome this problem by immobilizing RuBisCO into a microfluidic reactor, which demonstrates a continuous production of glucose precursor at 13.8 mu mol g(-1) RuBisCO min(-1) from CO2 and ribulose-1,5-bisphosphate. Experiments show that the RuBisCO immobilization significantly enhances enzyme stabilities (7.2 folds in storage stability, 6.7 folds in thermal stability), and also improves the reusability (90.4% activity retained after 5 cycles of reuse and 78.5% after 10 cycles). This work mimics the NPS pathway with scalable microreactors for continuous synthesis of glucose precursor using very small amount of RuBisCO. Although still far from industrial production, this work demonstrates artificial synthesis of basic food materials by replicating the light-independent reactions of NPS, which may hold the key to food crisis relief and future space colonization.
Publisher: Nature Publishing Group
Journal: Nature communications 
EISSN: 2041-1723
DOI: 10.1038/s41467-019-12089-6
Rights: Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third partymaterial in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly fromthe copyright holder. To view a copy of this license, visit
© The Author(s) 2019
The following publication Zhu, Y., Huang, Z., Chen, Q. et al. Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors. Nat Commun 10, 4049 (2019), 1-9 is available at
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