Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/81709
DC Field | Value | Language |
---|---|---|
dc.contributor | Department of Applied Biology and Chemical Technology | - |
dc.contributor | Department of Applied Physics | - |
dc.creator | Zhu, Y | - |
dc.creator | Huang, ZY | - |
dc.creator | Chen, Q | - |
dc.creator | Wu, Q | - |
dc.creator | Huang, XW | - |
dc.creator | So, PK | - |
dc.creator | Shao, LY | - |
dc.creator | Yao, Z | - |
dc.creator | Jia, YW | - |
dc.creator | Li, ZH | - |
dc.creator | Yu, WX | - |
dc.creator | Yang, Y | - |
dc.creator | Jian, AQ | - |
dc.creator | Sang, SB | - |
dc.creator | Zhang, WD | - |
dc.creator | Zhang, XM | - |
dc.date.accessioned | 2020-02-10T12:28:45Z | - |
dc.date.available | 2020-02-10T12:28:45Z | - |
dc.identifier.uri | http://hdl.handle.net/10397/81709 | - |
dc.language.iso | en | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.rights | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third partymaterial in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly fromthe copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | en_US |
dc.rights | © The Author(s) 2019 | en_US |
dc.rights | The following publication Zhu, Y., Huang, Z., Chen, Q. et al. Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors. Nat Commun 10, 4049 (2019), 1-9 is available at https://dx.doi.org/10.1038/s41467-019-12089-6 | en_US |
dc.title | Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.spage | 1 | - |
dc.identifier.epage | 9 | - |
dc.identifier.volume | 10 | - |
dc.identifier.doi | 10.1038/s41467-019-12089-6 | - |
dcterms.abstract | Food production in green crops is severely limited by low activity and poor specificity of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) in natural photosynthesis (NPS). This work presents a scientific solution to overcome this problem by immobilizing RuBisCO into a microfluidic reactor, which demonstrates a continuous production of glucose precursor at 13.8 mu mol g(-1) RuBisCO min(-1) from CO2 and ribulose-1,5-bisphosphate. Experiments show that the RuBisCO immobilization significantly enhances enzyme stabilities (7.2 folds in storage stability, 6.7 folds in thermal stability), and also improves the reusability (90.4% activity retained after 5 cycles of reuse and 78.5% after 10 cycles). This work mimics the NPS pathway with scalable microreactors for continuous synthesis of glucose precursor using very small amount of RuBisCO. Although still far from industrial production, this work demonstrates artificial synthesis of basic food materials by replicating the light-independent reactions of NPS, which may hold the key to food crisis relief and future space colonization. | - |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | Nature communications, 6 Sept. 2019, v. 10, 4049, p. 1-9 | - |
dcterms.isPartOf | Nature communications | - |
dcterms.issued | 2019 | - |
dc.identifier.isi | WOS:000484599200001 | - |
dc.identifier.eissn | 2041-1723 | - |
dc.identifier.artn | 4049 | - |
dc.description.validate | 202002 bcrc | - |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | OA_Scopus/WOS | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Zhu_Artificial_Synthesis_Glucose.pdf | 1.93 MB | Adobe PDF | View/Open |
Page views
93
Last Week
2
2
Last month
Citations as of Apr 14, 2024
Downloads
66
Citations as of Apr 14, 2024
SCOPUSTM
Citations
57
Citations as of Apr 12, 2024
WEB OF SCIENCETM
Citations
45
Citations as of Apr 18, 2024
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.