Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/81537
Title: Genetic characterization of blaCTX-M-55-bearing plasmids harbored by food-borne cephalosporin-resistant Vibrio parahaemolyticus Strains in China
Authors: Zheng, Z
Li, R 
Ye, L 
Chan, EWC 
Xia, X
Chen, S 
Keywords: blaCTX-M-55
Cephalosporin resistance
Conjugative plasmid
IncC plasmid
V. parahaemolyticus
Issue Date: 2019
Publisher: Frontiers Research Foundation
Source: Frontiers in microbiology, 2019, v. 10, no. JUN, 1338 How to cite?
Journal: Frontiers in microbiology 
Abstract: This study aims to investigate and compare the complete nucleotide sequences of the multidrug resistance plasmids pVb0267 and pVb0499, which were recovered from foodborne Vibrio parahaemolyticus isolates, and analyze the genetic environment of blaCTX-M-55 to provide insight into the dissemination mechanisms of this resistance element. Analysis of the sequences of plasmids pVb0267 (166, 467 bp) and pVb0499 (192, 739 bp) revealed that the backbones of these two plasmids exhibited a high degree of similarity with pR148, a recognized type 1a IncC plasmid recovered from Aeromonas hydrophila (99% identity). The resistance genes, found in both plasmids, included qacH, aadB, arr2, blaOXA-10, aadA1, sul1, tet(A), and blaCTX M-55, which were mostly arranged in a specific region designated ARI-A. Plasmid pVb0499 was found to possess a larger size of ARI-A than pVb0267, which lacked a mer determination region, a qnr A segment, an aacC3 gene and several mobility-encoding genes. Comparative analysis of resistance island (RI) of these plasmids and others revealed the potential evolution route of these RI sequences. In conclusion, plasmids harboring the blaCTX M-55 gene has been recovered in Vibrio parahaemolyticus strains of food origin. It is alarming to find that IncC plasmids harboring resistance islands are disseminating in aquatic bacterial strains. The continuous evolution of resistance genes in conjugative plasmid in aquatic bacteria could be due to bacterial adaption to aquaculture environment, where antibiotics were increasingly used.
URI: http://hdl.handle.net/10397/81537
ISSN: 1664-302X
DOI: 10.3389/fmicb.2019.01338
Rights: Copyright © 2019 Zheng, Li, Ye, Wai-chi Chan, Xia and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
The following publication Zheng Z, Li R, Ye L, Wai-chi Chan E, Xia X and Chen S (2019) Genetic Characterization of blaCTX-M-55 -Bearing Plasmids Harbored by Food-Borne Cephalosporin-Resistant Vibrio parahaemolyticus Strains in China. Front. Microbiol. 10:1338, is available at https://doi.org/10.3389/fmicb.2019.01338
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