Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/81128
Title: Xiao Qing Long Tang essential oil exhibits inhibitory effects on the release of pro-inflammatory mediators by suppressing NF-kappa B, AP-1, and IRF3 signalling in the lipopolysaccharide-stimulated RAW264.7 cells
Authors: Luo, G
Kong, J
Cheng, BCY 
Zhao, H
Fu, XQ
Yan, LS
Ding, Y
Liu, YL
Pan, SY
Zhang, SF
Zhang, Y
Issue Date: 2019
Publisher: Royal Society of Chemistry
Source: RSC advances, 2019, v. 9, no. 23, p. 12977-12989 How to cite?
Journal: RSC advances 
Abstract: Xiao Qing Long Tang (literally Minor blue dragon decoction in Chinese), a traditional Chinese formula, is prescribed to treat respiratory diseases. However, only few studies have been reported on its anti-inflammatory mechanisms. In this study, we investigated the inhibitory effects of Xiao Qing Long Tang essential oil on inflammatory mediators and explored the mechanisms of action of XQEO in the lipopolysaccharide (LPS)-stimulated RAW264.7 cells. XQEO was prepared via steam distillation and characterized by GC-MS analysis. MTT and Griess assays were used to measure cell viability and NO production, respectively. The mRNA expression and the production of LPS-induced pro-inflammatory cytokines (IL-1, IL-6, TNF-, and IL-10) and chemokines (MCP-1, Rantes, and MIP-1) were determined by real-time PCR and enzyme-linked immunosorbent assay, respectively. Furthermore, we determined the protein levels of the components of NF-B, AP-1 and IRF3 signalling by Western blotting. Immunofluorescence assay was used to estimate the nuclear translocation of NF-B, AP-1 and IRF3. The results showed that XQEO inhibited the secretion of NO and PGE2 and down-regulated the mRNA and protein levels of iNOS and COX-2. We also found that XQEO suppressed the LPS-induced overproduction of pro-inflammatory mediators. Moreover, XQEO inhibited the phosphorylation of NF-B/p65, AP-1/c-Jun, and IRF3 by suppressing their upstream kinases, such as MAPKs, TBK1, Akt, IKK/, and IB, reducing the LPS-induced NF-B, AP-1 and IRF3 translocation to the nucleus. These findings suggest that XQEO effectively suppresses the production of pro-inflammatory mediators possibly through the inhibition of NF-B, AP-1, and IRF3 signalling in the LPS-stimulated RAW264.7 cells.
URI: http://hdl.handle.net/10397/81128
EISSN: 2046-2069
DOI: 10.1039/c9ra01448a
Rights: This journal is © The Royal Society of Chemistry 2019
This article is licensed under a Creative Commons Attibution-NonCommercial 3.0 Unported Licence (https://creativecommons.org/licenses/by-nc/3.0/)
The following publication Luo, G., Kong, J., Cheng, B. C. Y., Zhao, H., Fu, X. Q., Yan, L. S., … & Zhang, Y. (2019). Xiao Qing Long Tang essential oil exhibits inhibitory effects on the release of pro-inflammatory mediators by suppressing NF-kappa B, AP-1, and IRF3 signalling in the lipopolysaccharide-stimulated RAW264.7 cells. RSC advances, 9(23), 12977-12989 is available at https://dx.doi.org/10.1039/c9ra01448a
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