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dc.contributor.authorLuo, Gen_US
dc.contributor.authorKong, Jen_US
dc.contributor.authorCheng, BCYen_US
dc.contributor.authorZhao, Hen_US
dc.contributor.authorFu, XQen_US
dc.contributor.authorYan, LSen_US
dc.contributor.authorDing, Yen_US
dc.contributor.authorLiu, YLen_US
dc.contributor.authorPan, SYen_US
dc.contributor.authorZhang, SFen_US
dc.contributor.authorZhang, Yen_US
dc.identifier.citationRSC advances, 2019, v. 9, no. 23, p. 12977-12989en_US
dc.description.abstractXiao Qing Long Tang (literally Minor blue dragon decoction in Chinese), a traditional Chinese formula, is prescribed to treat respiratory diseases. However, only few studies have been reported on its anti-inflammatory mechanisms. In this study, we investigated the inhibitory effects of Xiao Qing Long Tang essential oil on inflammatory mediators and explored the mechanisms of action of XQEO in the lipopolysaccharide (LPS)-stimulated RAW264.7 cells. XQEO was prepared via steam distillation and characterized by GC-MS analysis. MTT and Griess assays were used to measure cell viability and NO production, respectively. The mRNA expression and the production of LPS-induced pro-inflammatory cytokines (IL-1, IL-6, TNF-, and IL-10) and chemokines (MCP-1, Rantes, and MIP-1) were determined by real-time PCR and enzyme-linked immunosorbent assay, respectively. Furthermore, we determined the protein levels of the components of NF-B, AP-1 and IRF3 signalling by Western blotting. Immunofluorescence assay was used to estimate the nuclear translocation of NF-B, AP-1 and IRF3. The results showed that XQEO inhibited the secretion of NO and PGE2 and down-regulated the mRNA and protein levels of iNOS and COX-2. We also found that XQEO suppressed the LPS-induced overproduction of pro-inflammatory mediators. Moreover, XQEO inhibited the phosphorylation of NF-B/p65, AP-1/c-Jun, and IRF3 by suppressing their upstream kinases, such as MAPKs, TBK1, Akt, IKK/, and IB, reducing the LPS-induced NF-B, AP-1 and IRF3 translocation to the nucleus. These findings suggest that XQEO effectively suppresses the production of pro-inflammatory mediators possibly through the inhibition of NF-B, AP-1, and IRF3 signalling in the LPS-stimulated RAW264.7 cells.en_US
dc.description.sponsorshipCollege of Professional and Continuing Educationen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.ispartofRSC advancesen_US
dc.rightsThis journal is © The Royal Society of Chemistry 2019en_US
dc.rightsThis article is licensed under a Creative Commons Attibution-NonCommercial 3.0 Unported Licence (
dc.rightsThe following publication Luo, G., Kong, J., Cheng, B. C. Y., Zhao, H., Fu, X. Q., Yan, L. S., … & Zhang, Y. (2019). Xiao Qing Long Tang essential oil exhibits inhibitory effects on the release of pro-inflammatory mediators by suppressing NF-kappa B, AP-1, and IRF3 signalling in the lipopolysaccharide-stimulated RAW264.7 cells. RSC advances, 9(23), 12977-12989 is available at
dc.titleXiao Qing Long Tang essential oil exhibits inhibitory effects on the release of pro-inflammatory mediators by suppressing NF-kappa B, AP-1, and IRF3 signalling in the lipopolysaccharide-stimulated RAW264.7 cellsen_US
dc.typeJournal/Magazine Articleen_US
dc.description.validate201907 bcrc-
Appears in Collections:Journal/Magazine Article
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