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Title: Effect of lactamase inhibitors on the Biosensor Penp during the measurement of lactam antibiotics concentration
Authors: Soto, D
Silva, C
Ugalde, C
Wong, KY 
Leung, YC 
So, LY 
Andresen, M
Keywords: Biosensor
Lactamase inhibitors
Lactam antibiotics
Issue Date: 2019
Publisher: Molecular Diversity Preservation International (MDPI)
Source: Sensors, 1 Mar. 2019, v. 19, no. 5, 1237, p. 1-7 How to cite?
Journal: Sensors 
Abstract: PenP is a fluorescent biosensor of lactam antibiotics (LA). It is structurally derived from the mutant lactamase TEM-1 comprising the substitution E166C, where fluorescein is covalently linked to cysteine. The presence of LA in the medium produces a change in the intrinsic fluorescence level of the biosensor, and the integral of the fluorescence level over time correlates directly with the LA concentration. Previously, we have successfully used PenP to determine the concentration of lactam antibiotics in clinical samples. The use of lactamase inhibitors (LI) is a common strategy to enhance the effect of LA due to the inhibition of an important resistance mechanism of pathogenic microorganisms. Structurally, LI and LA share the common element of recognition of lactamases (the lactam ring), but they differ in the reversibility of the mechanism of interaction with said enzyme. Because the biological recognition domain of PenP is derived from a lactamase, LI is expected to interfere with the PenP detection capabilities. Surprisingly, this work provides evidence that the effect of LI is marginal in the determination of LA concentration mediated by PenP.
EISSN: 1424-8220
DOI: 10.3390/s19051237
Rights: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
The following publication Soto, D.; Silva, C.; Ugalde, C.; Wong, K.-Y.; Leung, Y.-C.; So, L.-Y.; Andresen, M. Effect of Lactamase Inhibitors on the Biosensor Penp during the Measurement of Lactam Antibiotics Concentration. Sensors 2019, 19, 1237, 7 pages is available at
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