Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/80749
DC Field | Value | Language |
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dc.contributor | Department of Applied Biology and Chemical Technology | - |
dc.creator | Soto, D | - |
dc.creator | Silva, C | - |
dc.creator | Ugalde, C | - |
dc.creator | Wong, KY | - |
dc.creator | Leung, YC | - |
dc.creator | So, LY | - |
dc.creator | Andresen, M | - |
dc.date.accessioned | 2019-05-28T01:09:06Z | - |
dc.date.available | 2019-05-28T01:09:06Z | - |
dc.identifier.uri | http://hdl.handle.net/10397/80749 | - |
dc.language.iso | en | en_US |
dc.publisher | Molecular Diversity Preservation International (MDPI) | en_US |
dc.rights | © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | en_US |
dc.rights | The following publication Soto, D.; Silva, C.; Ugalde, C.; Wong, K.-Y.; Leung, Y.-C.; So, L.-Y.; Andresen, M. Effect of Lactamase Inhibitors on the Biosensor Penp during the Measurement of Lactam Antibiotics Concentration. Sensors 2019, 19, 1237, 7 pages is available at https://dx.doi.org/10.3390/s19051237 | en_US |
dc.subject | Biosensor | en_US |
dc.subject | PenP | en_US |
dc.subject | Lactamase inhibitors | en_US |
dc.subject | Lactam antibiotics | en_US |
dc.subject | Use | en_US |
dc.title | Effect of lactamase inhibitors on the Biosensor Penp during the measurement of lactam antibiotics concentration | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.spage | 1 | - |
dc.identifier.epage | 7 | - |
dc.identifier.volume | 19 | - |
dc.identifier.issue | 5 | - |
dc.identifier.doi | 10.3390/s19051237 | - |
dcterms.abstract | PenP is a fluorescent biosensor of lactam antibiotics (LA). It is structurally derived from the mutant lactamase TEM-1 comprising the substitution E166C, where fluorescein is covalently linked to cysteine. The presence of LA in the medium produces a change in the intrinsic fluorescence level of the biosensor, and the integral of the fluorescence level over time correlates directly with the LA concentration. Previously, we have successfully used PenP to determine the concentration of lactam antibiotics in clinical samples. The use of lactamase inhibitors (LI) is a common strategy to enhance the effect of LA due to the inhibition of an important resistance mechanism of pathogenic microorganisms. Structurally, LI and LA share the common element of recognition of lactamases (the lactam ring), but they differ in the reversibility of the mechanism of interaction with said enzyme. Because the biological recognition domain of PenP is derived from a lactamase, LI is expected to interfere with the PenP detection capabilities. Surprisingly, this work provides evidence that the effect of LI is marginal in the determination of LA concentration mediated by PenP. | - |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | Sensors, 1 Mar. 2019, v. 19, no. 5, 1237, p. 1-7 | - |
dcterms.isPartOf | Sensors | - |
dcterms.issued | 2019 | - |
dc.identifier.isi | WOS:000462540400256 | - |
dc.identifier.pmid | 30870966 | - |
dc.identifier.eissn | 1424-8220 | - |
dc.identifier.artn | 1237 | - |
dc.description.validate | 201905 bcrc | - |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | OA_IR/PIRA | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
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Soto_Lactamase_Biosensor_Lactam.pdf | 544.63 kB | Adobe PDF | View/Open |
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