Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/80062
Title: Purification and in vitro antioxidant activities of tellurium-containing phycobiliproteins from tellurium-enriched Spirulina platensis
Authors: Yang, F
Wong, KH 
Yang, Y
Li, X
Jiang, J
Zheng, W
Wu, H
Chen, T
Keywords: Allophycocyanin
Antioxidant activity
Phycocyanin
Purification
Tellurium
Issue Date: 2014
Publisher: Dove Medical Press
Source: Drug design, development and therapy, 2014, v. 8, p. 1789-1800 How to cite?
Journal: Drug design, development and therapy 
Abstract: Tellurium-containing phycocyanin (Te-PC) and allophycocyanin (Te-APC), two organic tellurium (Te) species, were purified from tellurium-enriched Spirulina platensis by a fast protein liquid chromatographic method. It was found that the incorporation of Te into the peptides enhanced the antioxidant activities of both phycobiliproteins. With fractionation by ammonium sulfate precipitation and hydroxylapatite chromatography, Te-PC and Te-APC could be effectively separated with high purity, and Te concentrations were 611.1 and 625.3 µg g-1 protein in Te-PC and Te-APC, respectively. The subunits in the proteins were identified by using MALDI-TOF-TOF mass spectrometry. Te incorporation enhanced the antioxidant activities of both phycobiliproteins, as examined by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid assay. Moreover, Te-PC and Te-APC showed dose-dependent protection on erythrocytes against the water-soluble free radical initiator 2,2'-azo(2-asmidinopropane)dihydrochloride-induced hemolysis. In the hepatoprotective model, apoptotic cell death and nuclear condensation induced by tert-butyl hydroperoxide in HepG2 cells was significantly attenuated by Te-PC and Te-APC. Taken together, these results suggest that Te-PC and Te-APC are promising Te-containing proteins with application potential for treatment of diseases related to oxidative stress.
URI: http://hdl.handle.net/10397/80062
EISSN: 1177-8881
DOI: 10.2147/DDDT.S62530
Rights: © 2014 Yang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License. The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions beyond the scope of the License are administered by Dove Medical Press Limited. Information on how to request permission may be found at: http://www.dovepress.com/permissions.php
The following publication Yang, F., Wong, K. -., Yang, Y., Li, X., Jiang, J., Zheng, W., . . . Chen, T. (2014). Purification and in vitro antioxidant activities of tellurium-containing phycobiliproteins from tellurium-enriched spirulina platensis. Drug Design, Development and Therapy, 8, 1789-1800 is available at https://dx.doi.org/10.2147/DDDT.S62530
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