Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/78628
Title: Insulin resistance enhances the mitogen-activated protein kinase signaling pathway in ovarian granulosa cells
Authors: Kong, LH
Wang, Q
Jin, JW
Xiang, Z 
Chen, TY
Shen, SM
Wang, HW
Gao, Q
Wang, Y
Issue Date: 2017
Publisher: Public Library of Science
Source: PLoS one, 10 Nov. 2017, v. 12, no. 11, e0188029 How to cite?
Journal: PLoS one 
Abstract: The ovary is the main regulator of female fertility. Granulosa cell dysfunction may be involved in various reproductive endocrine disorders. Here we investigated the effect of insulin resistance on the metabolism and function of ovarian granulosa cells, and dissected the functional status of the mitogen-activated protein kinase signaling pathway in these cells. Our data showed that dexamethasone-induced insulin resistance in mouse granulosa cells reduced insulin sensitivity, accompanied with an increase in phosphorylation of p44/42 mitogen-activated protein kinase. Furthermore, up-regulation of cytochrome P450 subfamily 17 and testosterone and down-regulation of progesterone were observed in insulin-resistant mouse granulosa cells. Inhibition of p44/42 mitogen-activated protein kinase after induction of insulin resistance in mouse granulosa cells decreased phosphorylation of p44/42 mitogen- activated protein kinase, downregulated cytochrome P450 subfamily 17 and lowered progesterone production. This insulin resistance cell model can successfully demonstrate certain mechanisms such as hyperandrogenism, which may inspire a new strategy for treating reproductive endocrine disorders by regulating cell signaling pathways.
URI: http://hdl.handle.net/10397/78628
EISSN: 1932-6203
DOI: 10.1371/journal.pone.0188029
Rights: © 2017 Kong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The following publication Kong, L., Wang, Q., Jin, J., Xiang, Z., Chen, T., Shen, S., . . . Wang, Y. (2017). Insulin resistance enhances the mitogen-activated protein kinase signaling pathway in ovarian granulosa cells. PLoS ONE, 12(11), e0188029 is available at https://doi.org/10.1371/journal.pone.0188029
Appears in Collections:Journal/Magazine Article

Files in This Item:
File Description SizeFormat 
Kong_Insulin_Resistance_Mitogen-activated.pdf1.87 MBAdobe PDFView/Open
Access
View full-text via PolyU eLinks SFX Query
Show full item record
PIRA download icon_1.1View/Download Contents

SCOPUSTM   
Citations

1
Citations as of Dec 15, 2018

Page view(s)

22
Citations as of Dec 10, 2018

Download(s)

3
Citations as of Dec 10, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.