Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/70708
Title: (-)-Epiafzelechin protects against ovariectomy-induced bone loss in adult mice and modulate osteoblastic and osteoclastic functions in vitro
Authors: Wong, KC 
Cao, SS 
Dong, XL 
Law, MC 
Chan, TH 
Wong, MS 
Issue Date: 2017
Source: Nutrients, May 2017, v. 9, no. 5, 530, p. 1-14
Abstract: The present study was designed to characterize the bone protective effects of (-)-epiafzelechin (EAF), a flavan-3-ol, in mature ovariectomized mice model and its ability to stimulate osteoblastic activity and inhibit osteoclastic activity. Mature C57BL/6 mice (three to four months old) were either ovariectomised (OVX) or sham-operated and subjected to treatment (vehicle, 17 beta-oestradiol (E2, 200 mu g/kg/day) or EAF ( 500 mu g/kg/day) orally for six weeks. EAF and E2 significantly reduced urinary calcium (Ca) excretion, serum osteocalcin (OCN), and urinary deoxy-pyridinoline (DPD); increased bone mineral density (BMD); and improved micro-architectural properties in OVX mice. EAF significantly increased cell viability, alkaline phosphatise (ALP) activity, and collagen content, as well as runt-related transcriptional factor 2 (Runx2) mRNA expression in murine osteoblastic MC3T3-E1 cells. In addition, EAF significantly reduced the viability of osteoclast precursor murine leukemia monocyte RAW264.7 cells and tartrate-resistant acid phosphatase (TRAP) activities in mature osteoclastic RAW 264.7 cells. EAF is a bioactive flavan-3-ol that protects estrogen deficiency-induced bone loss in OVX mice and exerts direct modulating effects in bone cells in vitro.
Keywords: (-)-epiafzelechin
Flavan-3-ol
Ovariectomised
Osteogenesis
Osteoclastogenesis
Trabecular bone
Publisher: Molecular Diversity Preservation International (MDPI)
Journal: Nutrients 
ISSN: 2072-6643
DOI: 10.3390/nu9050530
Rights: © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
The following publication Wong, K. C., Cao, S. S., Dong, X. L., Law, M. C., Chan, T. H., & Wong, M. S. (2017). (-)-Epiafzelechin protects against ovariectomy-induced bone loss in adult mice and modulate osteoblastic and osteoclastic functions in vitro. Nutrients, 9(5), (Suppl. ), 530, - is available athttps://dx.doi.org/10.3390/nu9050530
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