Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/68572
Title: C-terminal truncated hepatitis B virus X protein regulates tumorigenicity, self-renewal and drug resistance via STAT3/Nanog signaling pathway
Authors: Ching, RHH
Sze, KMF
Lau, EYT
Chiu, YT
Lee, JMF
Ng, IOL
Lee, TKW 
Keywords: Truncated HBx
HCC
Nanog
Stat3
Stemness
Hepatocellular carcinoma
Issue Date: 2017
Publisher: Impact Journals LLC
Source: Oncotarget, 2017, v. 8, no. 14, p. 23507-23516 How to cite?
Journal: Oncotarget 
Abstract: Hepatitis B virus (HBV) is a major risk factor of chronic liver disease and hepatocellular carcinoma (HCC). Random integration of HBV DNA into the host genome is frequent in HCC leading to truncation of the HBV DNA, particularly at the C-terminal end of the HBV X protein (HBx). C-terminally truncated HBx (HBx-Delta C) has been implicated in playing a pro-oncogenic role in hepatocarcinogenesis. However, the mechanism whereby HBx-Delta C1 contributes to hepatocarcinogenesis remains unclear. In this study, we investigated the functional role of HBx-Delta C1 in regulating liver cancer stem cell (CSC) properties. Using Tet-on inducible system, we found that HBx-Delta C1 enhanced CSC properties including self-renewal, tumorigenicity, chemoresistance, migration and expression of liver CSC markers, when compared with the full-length HBx counterpart and vector control. Interestingly, HBx-Delta C1 conferred resistance in HCC cells towards sorafenib treatment through suppression of apoptotic cascade. In addition, HBx-Delta C1 upregulated a panel of stemness genes, in which Nanog was found to be among the most significant one in both trasnfected cell lines. Consistently, Nanog was upregulated in human HCC samples which had HBx-Delta C1 expression. Furthermore, the induction of CSC properties by HBx-Delta C1 was via the Stat3/Nanog pathway, as administration of Stat3 inhibitor abolished the HBx-Delta C1-induced self-renewing capacity. In conclusion, our data suggest that HBx-Delta C1 enhances liver CSCs properties through Stat3/Nanog cascade, and provide a new insight for the therapeutic intervention for HBV-related HCC.
URI: http://hdl.handle.net/10397/68572
ISSN: 1949-2553
DOI: 10.18632/oncotarget.15183
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

Page view(s)

133
Last Week
83
Last month
Checked on Sep 17, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.