Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/68572
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dc.contributor.authorChing, RHHen_US
dc.contributor.authorSze, KMFen_US
dc.contributor.authorLau, EYTen_US
dc.contributor.authorChiu, YTen_US
dc.contributor.authorLee, JMFen_US
dc.contributor.authorNg, IOLen_US
dc.contributor.authorLee, TKWen_US
dc.date.accessioned2017-08-29T04:41:28Z-
dc.date.available2017-08-29T04:41:28Z-
dc.date.issued2017-
dc.identifier.citationOncotarget, 2017, v. 8, no. 14, p. 23507-23516en_US
dc.identifier.issn1949-2553en_US
dc.identifier.urihttp://hdl.handle.net/10397/68572-
dc.description.abstractHepatitis B virus (HBV) is a major risk factor of chronic liver disease and hepatocellular carcinoma (HCC). Random integration of HBV DNA into the host genome is frequent in HCC leading to truncation of the HBV DNA, particularly at the C-terminal end of the HBV X protein (HBx). C-terminally truncated HBx (HBx-Delta C) has been implicated in playing a pro-oncogenic role in hepatocarcinogenesis. However, the mechanism whereby HBx-Delta C1 contributes to hepatocarcinogenesis remains unclear. In this study, we investigated the functional role of HBx-Delta C1 in regulating liver cancer stem cell (CSC) properties. Using Tet-on inducible system, we found that HBx-Delta C1 enhanced CSC properties including self-renewal, tumorigenicity, chemoresistance, migration and expression of liver CSC markers, when compared with the full-length HBx counterpart and vector control. Interestingly, HBx-Delta C1 conferred resistance in HCC cells towards sorafenib treatment through suppression of apoptotic cascade. In addition, HBx-Delta C1 upregulated a panel of stemness genes, in which Nanog was found to be among the most significant one in both trasnfected cell lines. Consistently, Nanog was upregulated in human HCC samples which had HBx-Delta C1 expression. Furthermore, the induction of CSC properties by HBx-Delta C1 was via the Stat3/Nanog pathway, as administration of Stat3 inhibitor abolished the HBx-Delta C1-induced self-renewing capacity. In conclusion, our data suggest that HBx-Delta C1 enhances liver CSCs properties through Stat3/Nanog cascade, and provide a new insight for the therapeutic intervention for HBV-related HCC.en_US
dc.description.sponsorshipDepartment of Applied Biology and Chemical Technologyen_US
dc.language.isoenen_US
dc.publisherImpact Journals LLCen_US
dc.relation.ispartofOncotargeten_US
dc.subjectTruncated HBxen_US
dc.subjectHCCen_US
dc.subjectNanogen_US
dc.subjectStat3en_US
dc.subjectStemnessen_US
dc.subjectHepatocellular carcinomaen_US
dc.titleC-terminal truncated hepatitis B virus X protein regulates tumorigenicity, self-renewal and drug resistance via STAT3/Nanog signaling pathwayen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage23507en_US
dc.identifier.epage23516en_US
dc.identifier.volume8en_US
dc.identifier.issue14en_US
dc.identifier.doi10.18632/oncotarget.15183en_US
dc.identifier.isiWOS:000398211100102-
dc.identifier.pmid28186991-
dc.source.typeArticleen
item.fulltextFull Text (via PolyU elinks)-
crisitem.author.deptDepartment of Applied Biology and Chemical Technology-
crisitem.author.facultyFaculty of Applied Science and Textiles-
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