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Title: The role of miR-497-5p in myofibroblast differentiation of LR-MSCs and pulmonary fibrogenesis
Authors: Chen, X
Shi, CW
Wang, C
Liu, WL
Chu, YH
Zou, X 
Hu, KB
Dong, P
Han, XD
Issue Date: 2017
Publisher: Nature Publishing Group
Source: Scientific reports, 2017, v. 7, 40958, p. 1-13 How to cite?
Journal: Scientific reports 
Abstract: Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive and fatal fibrotic lung disease characterized by profound changes in stem cell differentiation, epithelial cell phenotypes and fibroblast proliferation. In our study, we found that miR-497-5p was significantly upregulated both during myofibroblast differentiation of lung resident mesenchymal stem cells (LR-MSCs) and in the lung tissues of a pulmonary fibrosis model. In addition, as determined by luciferase assays and Western blot analysis, reversion-inducing cysteine-rich protein with kazal motifs (Reck) was identified to be one of the target genes of miR-497-5p, and Reck could suppress the expression of matrix metalloproteinase-2 (Mmp2) and Mmp9, which could activate latent transforming growth factor-β1 (TGF-β1). To test the potential therapeutic significance of this miRNA, we modulated the expression of miR-497-5p in LR-MSCs and relevant animal models. The results demonstrated that upregulation of miR-497-5p could induce LR-MSCs to differentiate into myofibroblasts and promote pulmonary fibrogenesis, while inhibition of its expression could effectively retard these processes. In conclusion, our work supports that controlling pulmonary fibrogenesis via inhibition of miR-497-5p expression may provide a potential therapeutic strategy for IPF.
EISSN: 2045-2322
DOI: 10.1038/srep40958
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
© The Author(s) 2017
The following publication Chen, X., Shi, C., Wang, C., Liu, W., Chu, Y., Xiang, Z., ... & Han, X. (2017). The role of miR-497-5p in myofibroblast differentiation of LR-MSCs and pulmonary fibrogenesis. Scientific reports, 7, 40958 is available at
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