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Title: Differential expression of lipid metabolism related genes in HepG2 cells treated with neotoralactone
Other Titles: 新決明內脂誘導HepG2細胞內脂質代謝相關基因的差異表達
Authors: Au, TS
Yang, DJ
Zhang, Y
Wong, MS 
Chen, SL
Fong, WF
Xiao, PG
Yang, MS
Keywords: Cassia obtusifolia
cDNA microarray
Issue Date: 2003
Publisher: 中國醫學科學院 ; 中國協和醫科大學藥物硏究所
Source: 藥學學報 (Acta pharmaceutica Sinica), 2003, v. 38, no. 1, p. 62-68 How to cite?
Journal: 藥學學報 (Acta pharmaceutica Sinica) 
Abstract: Aim To study the biological effect and molecular mechanism of neotoralactone on lipid metabolism in HepG2 cells.
Methods Animal experiments were carried out to examine the biological effects of the compound. Differentiation assay was carried out to determine the effect of the compound on the 3T3-L1 preadipocyte. Finally, a high-density cDNA microarray containing about 10 000 human genes and ESTs were used to analyze the gene expression profiles of the HepG2 cells with and without neotoralactone treatment.
Results Preliminary results showed that neotoralactone can decrease the increase of body weight of rats fed with high nutrient diet. As the differentiation assay showed that neotoralactone has minimal effect on the obese 3T3-L1 preadipocyte cell line, HepG2 heptocarcinoma cell line was used as the model system because one of the proposed mechanisms of weight reduction effect of Cassia obtusifolia is the alteration of lipid metabolism primarily taken place in the liver. The results showed that neotoralactone regulates a panel of genes related to lipid metabolism.
Conclusion The effects of neotoralactone may be due to the regulation of a panel of important genes related to lipid metabolism.
ISSN: 0513-4870
Rights: © 2003 中国学术期刊电子杂志出版社。本内容的使用仅限于教育、科研之目的。
© 2003 China Academic Journal Electronic Publishing House. It is to be used strictly for educational and research purposes.
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