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Title: Genotyping and species identification of Fritillaria by DNA chips
Other Titles: DNA芯片技術用于貝母的基因分型和種類鑒別
Authors: Tsoi, PY
Woo, HS
Wong, MS 
Chen, SL
Fong, WF
Xiao, PG
Yang, MS
Keywords: DNA chip
Species identification
Issue Date: 2003
Publisher: 中國醫學科學院 ; 中國協和醫科大學藥物硏究所
Source: 藥學學報 (Acta pharmaceutica Sinica), 2003, v. 38, no. 3, p. 185-190 How to cite?
Journal: 藥學學報 (Acta pharmaceutica Sinica) 
Abstract: AIM: To investigate the genetic polymorphism of several species of Fritillaria and to develop a DNA chip for the genotyping and identification of the origin of various species of Fritillaria at molecular level.
METHODS: Genomic DNA from bulbs of several Fritillaria species was extracted and the polymorphisms of the D2 and D3 regions inside the 26S rDNA gene were identified by direct sequencing. Oligonucleotide probes specific for these polymorphisms were designed and printed on the poly-lysine coated slides to prepare the DNA chip. PCR products from the Fritillaria species were labeled with fluorescence by incorporation of dye-labeled dideoxyribonucleotides and hybridized to the immobilized probes on the chip.
RESULTS: The polymorphisms were used as markers for discrimination among various species. Specific oligonucleotide probes were designed and immobilized on a DNA chip. Differentiation of the various Fritillaria species was accomplished based on hybridization of fluorescent labeled PCR products with the DNA chip.
CONCLUSION: The results demonstrated the reliability of using DNA chips to identify different species of Fritillaria, and the DNA chip technology can provide a rapid, high throughput tool for genotyping and quality assurance of the plant species verification.
ISSN: 0513-4870
Rights: © 2003 中国学术期刊电子杂志出版社。本内容的使用仅限于教育、科研之目的。
© 2003 China Academic Journal Electronic Publishing House. It is to be used strictly for educational and research purposes.
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