Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/63768
DC Field | Value | Language |
---|---|---|
dc.contributor | Department of Applied Biology and Chemical Technology | - |
dc.creator | Tsoi, PY | - |
dc.creator | Woo, HS | - |
dc.creator | Wong, MS | - |
dc.creator | Chen, SL | - |
dc.creator | Fong, WF | - |
dc.creator | Xiao, PG | - |
dc.creator | Yang, MS | - |
dc.date.accessioned | 2017-02-09T08:30:32Z | - |
dc.date.available | 2017-02-09T08:30:32Z | - |
dc.identifier.issn | 0513-4870 | - |
dc.identifier.uri | http://hdl.handle.net/10397/63768 | - |
dc.language.iso | en | en_US |
dc.publisher | 中國醫學科學院 ; 中國協和醫科大學藥物硏究所 | en_US |
dc.rights | © 2003 中国学术期刊电子杂志出版社。本内容的使用仅限于教育、科研之目的。 | en_US |
dc.rights | © 2003 China Academic Journal Electronic Publishing House. It is to be used strictly for educational and research purposes. | en_US |
dc.subject | DNA chip | en_US |
dc.subject | Genotyping | en_US |
dc.subject | Fritillaria | en_US |
dc.subject | Species identification | en_US |
dc.title | Genotyping and species identification of Fritillaria by DNA chips | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.spage | 185 | - |
dc.identifier.epage | 190 | - |
dc.identifier.volume | 38 | - |
dc.identifier.issue | 3 | - |
dcterms.abstract | AIM: To investigate the genetic polymorphism of several species of Fritillaria and to develop a DNA chip for the genotyping and identification of the origin of various species of Fritillaria at molecular level. | - |
dcterms.abstract | METHODS: Genomic DNA from bulbs of several Fritillaria species was extracted and the polymorphisms of the D2 and D3 regions inside the 26S rDNA gene were identified by direct sequencing. Oligonucleotide probes specific for these polymorphisms were designed and printed on the poly-lysine coated slides to prepare the DNA chip. PCR products from the Fritillaria species were labeled with fluorescence by incorporation of dye-labeled dideoxyribonucleotides and hybridized to the immobilized probes on the chip. | - |
dcterms.abstract | RESULTS: The polymorphisms were used as markers for discrimination among various species. Specific oligonucleotide probes were designed and immobilized on a DNA chip. Differentiation of the various Fritillaria species was accomplished based on hybridization of fluorescent labeled PCR products with the DNA chip. | - |
dcterms.abstract | CONCLUSION: The results demonstrated the reliability of using DNA chips to identify different species of Fritillaria, and the DNA chip technology can provide a rapid, high throughput tool for genotyping and quality assurance of the plant species verification. | - |
dcterms.accessRights | open access | en_US |
dcterms.alternative | DNA芯片技術用于貝母的基因分型和種類鑒別 | - |
dcterms.bibliographicCitation | 藥學學報 (Acta pharmaceutica Sinica), 2003, v. 38, no. 3, p. 185-190 | - |
dcterms.isPartOf | 藥學學報 (Acta pharmaceutica Sinica) | - |
dcterms.issued | 2003 | - |
dc.identifier.rosgroupid | r14571 | - |
dc.description.ros | 2002-2003 > Academic research: refereed > Publication in refereed journal | - |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | OA_IR/PIRA | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
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File | Description | Size | Format | |
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r14571.pdf | 236.46 kB | Adobe PDF | View/Open |
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