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Title: Production of Poly-3-Hydroxybutyrate (PHB) by fermentation of active sludge from food plant (II)
Other Title: 利用食品工廠活性污泥發酵生產PHB的培養基配方研究(二)
Authors: Chen, R
Yang, YH
Yu, PHF
Issue Date: 2001
Source: 农业环境科学学报 (Journal of agro-environmental science), 2001, v. 20, no. 6, p. 424-428
Abstract: A media for fermentation of active sludge in wastewater treatment plant of Guangzhou Siming Yantang Diary Corporation were selected through L9(34) table of orthogonal arrays by designing nine different prescriptions for 3 elements, i.e. C, N and P, with 3 levels and the extracts from the cells of the sludge were tested through a series of physical and chemical examinations in present study. The results showed that the optimum culture medium for fermentation gained through various evaluations contained glucose of 8 g, (NH4)2SO4 of 0.25 g, and 0.25 g of KH2PO4 /liter of water. The extracts from the cells were qualitatively identified to be mainly poly-3-hydroxybutyrate by 1H NMR tests, while their concentration was quantitatively to be over 70.61% by GC test. It was also found that their melting points were between 182.2—188.9℃, higher than PHB standard.
Keywords: Active sludge
Culture of fermentation
Optimization of preparation
Publisher: 中國學術期刊(光盤版)電子雜誌社
Journal: 农业环境科学学报 (Journal of agro-environmental science) 
ISSN: 1672-2043
Rights: © 2001 中国学术期刊电子杂志出版社。本内容的使用仅限于教育、科研之目的。
© 2001 China Academic Journal Electronic Publishing House. It is to be used strictly for educational and research purposes.
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