Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/60161
Title: Effects of swim training on the expression of gastrocnemius’ iron transport proteins in rats
Other Titles: 游泳训练对大鼠腓肠肌铁代谢相关蛋白表达的影响
Authors: Liu, YQ
Chang, YZ
Qian, ZM
Liu, JG
Duan, XL
Keywords: Gastrocnemius
Non-heam iron
Ferroportin 1
Divalent metal transporter 1
Rats
Issue Date: 2005
Publisher: 人民体育出版社
Source: 中国运动医学杂志 (Chinese journal of sports medicine), Mar. 2005, v. 24, no. 2, p. 147-151 How to cite?
Journal: 中国运动医学杂志 (Chinese journal of sports medicine) 
Abstract: 目的: 研究游泳训练后大鼠腓肠肌二价金属离子转运体 1 (divalentmetaltransporter1 ,DMT1 )、转铁蛋白受体 (transferrinreceptor,TfR)及膜铁转运蛋白 1 (ferroportin1 ,FPN1 )表达的变化 ,探究运动对腓肠肌铁代谢的影响机制。
方法: 4 0只雌性SD大鼠随机分为对照组和游泳训练组 ,分别于游泳训练 5周及 1 0周后取材 ,测定血清铁状态、腓肠肌非血红素铁含量 ,采用WesternBlot方法检测腓肠肌铁代谢相关蛋白表达的变化。
结果: (1 )游泳训练 5周及 1 0周组大鼠血清铁、Hb及RBC均较对照组显著升高 ,游泳运动 1 0周组大鼠腓肠肌非血红素铁含量显著增加 (P <0 . 0 1 ) ;(2 )游泳训练组大鼠腓肠肌DMT1 (IRE)和TfR表达增加 ,FPN1表达减少 (P <0 . 0 5 ) ,而DMT1 (non -IRE)表达无明显变化。
结论: 游泳训练可以增加腓肠肌非血红素铁含量 ,促进机体铁动员。这可能与腓肠肌细胞膜上向胞内转运铁的TfR和DMT1(IRE)蛋白表达增加 ,而向胞外转运铁的FPN1表达下降有关。
Objective: To investigate the effects of swim training on the expression of divalent metal transporter 1(DMT1), transferrin receptor(TfR) and ferroportin 1(FRN1) in rats’ gastrocnemius.
Methods: 40 female SD rats were randomly divided into two groups: control group(CG)and exercise group(EG), and were sampled after 5 and 10-week swimming. Determined serum iron status and non-heam iron of gastrocnemius. Observed the expression of TfR, DMT1 and FPN1 by Western Blot.
Results: (1)The serum iron, Hb and RBC were significantly higher than those in control group. The gastrocnemius’ non-heam iron of exercise group were significantly increased after 10-week training(P<0.01). (2) The expression of DMT1 (IRE) and TfR of exercise group was increased, but FPN1 expression was decreased, and DMT1 (non-IRE) had no change.
Conclusion: Suitable training could improve body iron homeostasis, increase gastrocnemius’ non-heam iron. The mechanism of changes were that the expression of DMT1 (IRE) and TfR were increased but that of FPN1 was decreased.
URI: http://hdl.handle.net/10397/60161
ISSN: 1000-6710
DOI: 10.16038/j.1000-6710.2005.02.004
Rights: © 2005 中国学术期刊电子杂志出版社。本内容的使用仅限于教育、科研之目的。
© 2005 China Academic Journal Electronic Publishing House. It is to be used strictly for educational and research purposes.
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