Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/43547
Title: A novel PCR-based approach for accurate identification of vibrio parahaemolyticus
Authors: Li, R
Chiou, J 
Chan, EWC
Chen, S 
Keywords: BlaCARB-17
Molecular detection
PCR
Vibrio parahaemolyticus
Issue Date: 2016
Publisher: Frontiers Research Foundation
Source: Frontiers in microbiology, 2016, v. 7, no. JAN, 00044 How to cite?
Journal: Frontiers in microbiology 
Abstract: A PCR-based assay was developed for more accurate identification of Vibrio parahaemolyticus through targeting the blaCARB-17 like element, an intrinsic β-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. Homologous analysis showed that blaCARB-17 like genes were more conservative than the tlh, toxR and atpA genes, the genetic markers commonly used as detection targets in identification of V. parahaemolyticus. Our data showed that this blaCARB-17-specific PCR-based detection approach consistently achieved 100% specificity, whereas PCR targeting the tlh and atpA genes occasionally produced false positive results. Furthermore, a positive result of this test is consistently associated with an intrinsic ampicillin resistance phenotype of the test organism, presumably conferred by the products of blaCARB-17 like genes. We envision that combined analysis of the unique genetic and phenotypic characteristics conferred by blaCARB-17 shall further enhance the detection specificity of this novel yet easy-to-use detection approach to a level superior to the conventional methods used in V. parahaemolyticus detection and identification.
URI: http://hdl.handle.net/10397/43547
ISSN: 1664-302X
DOI: 10.3389/fmicb.2016.00044
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