Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/43547
DC Field | Value | Language |
---|---|---|
dc.contributor | Department of Applied Biology and Chemical Technology | - |
dc.creator | Li, R | - |
dc.creator | Chiou, J | - |
dc.creator | Chan, EWC | - |
dc.creator | Chen, S | - |
dc.date.accessioned | 2016-06-07T06:16:40Z | - |
dc.date.available | 2016-06-07T06:16:40Z | - |
dc.identifier.issn | 1664-302X | en_US |
dc.identifier.uri | http://hdl.handle.net/10397/43547 | - |
dc.language.iso | en | en_US |
dc.publisher | Frontiers Research Foundation | en_US |
dc.rights | Copyright © 2016 Li, Chiou, Chan and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | en_US |
dc.rights | The following publication Li R, Chiou J, Chan EW-C and Chen S (2016) A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus. Front. Microbiol. 7:44,1-5 is available at https://dx.doi.org/10.3389/fmicb.2016.00044 | en_US |
dc.subject | BlaCARB-17 | en_US |
dc.subject | Molecular detection | en_US |
dc.subject | PCR | en_US |
dc.subject | Vibrio parahaemolyticus | en_US |
dc.title | A novel PCR-based approach for accurate identification of vibrio parahaemolyticus | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.spage | 1 | en_US |
dc.identifier.epage | 7 | en_US |
dc.identifier.volume | 7 | en_US |
dc.identifier.doi | 10.3389/fmicb.2016.00044 | en_US |
dcterms.abstract | A PCR-based assay was developed for more accurate identification of Vibrio parahaemolyticus through targeting the blaCARB-17 like element, an intrinsic β-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. Homologous analysis showed that blaCARB-17 like genes were more conservative than the tlh, toxR and atpA genes, the genetic markers commonly used as detection targets in identification of V. parahaemolyticus. Our data showed that this blaCARB-17-specific PCR-based detection approach consistently achieved 100% specificity, whereas PCR targeting the tlh and atpA genes occasionally produced false positive results. Furthermore, a positive result of this test is consistently associated with an intrinsic ampicillin resistance phenotype of the test organism, presumably conferred by the products of blaCARB-17 like genes. We envision that combined analysis of the unique genetic and phenotypic characteristics conferred by blaCARB-17 shall further enhance the detection specificity of this novel yet easy-to-use detection approach to a level superior to the conventional methods used in V. parahaemolyticus detection and identification. | - |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | Frontiers in microbiology, 28 Jan. 2016, v. 7, 44, p. 1-5 | - |
dcterms.isPartOf | Frontiers in microbiology | - |
dcterms.issued | 2016-01-28 | - |
dc.identifier.isi | WOS:000368817900001 | - |
dc.identifier.scopus | 2-s2.0-84957973608 | - |
dc.identifier.artn | 44 | en_US |
dc.identifier.rosgroupid | 2015001034 | - |
dc.description.ros | 2015-2016 > Academic research: refereed > Publication in refereed journal | en_US |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | OA_IR/PIRA | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
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Li_Novel_PCR-based_Approach.pdf | 528.36 kB | Adobe PDF | View/Open |
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