Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/36350
Title: Isolation and structure characterization of an antioxidative glycopeptide from mycelial culture broth of a medicinal fungus
Authors: Wu, JY 
Chen, X
Siu, KC
Keywords: Cordyceps sinensis
Glycopeptide
Structure
Antioxidant
Cell protection
Issue Date: 2014
Publisher: Molecular Diversity Preservation International (MDPI)
Source: International journal of molecular sciences, 2014, v. 15, no. 10, p. 17318-17332 How to cite?
Journal: International journal of molecular sciences 
Abstract: A novel glycopeptide (Cs-GP1) with an average molecular weight (Mw) of 6.0 kDa was isolated and purified by column chromatography from the lower Mw fraction of exopolysaccharide (EPS) produced by a medicinal fungus Cordyceps sinensis Cs-HK1. Its carbohydrate moiety was mainly composed of glucose and mannose at 3.2:1.0 mole ratio, indicating an O-linked glycopeptide. The peptide chain contained relatively high mole ratios of aspartic acid, glutamic acid and glycine (3.3-3.5 relative to arginine) but relatively low ratios of tyrosine and histidine. The peptide chain sequence analyzed after trypsin digestion by LC-MS was KNGIFQFGEDCAAGSISHELGGFREFREFLKQAGLE. Cs-GP1 exhibited remarkable antioxidant capacity with a Trolox equivalent antioxidant capacity of 1183.8 mu mol/g and a ferric reducing ability of 611.1 mu mol Fe(II)/g, and significant protective effect against H2O2-induced PC12 cell injury at a minimum dose of 10 mu g/mL. This is the first report on the structure and bioactivity of an extracellular glycopeptide from the Cordyceps species.
URI: http://hdl.handle.net/10397/36350
ISSN: 1422-0067 (print)
1661-6596 (online)
DOI: 10.3390/ijms151017318
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