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Title: | Isolation and structure characterization of an antioxidative glycopeptide from mycelial culture broth of a medicinal fungus | Authors: | Wu, JY Chen, X Siu, KC |
Issue Date: | 2014 | Source: | International journal of molecular sciences, Oct. 2014, v. 15, no. 10, p. 17318-17332 | Abstract: | A novel glycopeptide (Cs-GP1) with an average molecular weight (Mw) of 6.0 kDa was isolated and purified by column chromatography from the lower Mw fraction of exopolysaccharide (EPS) produced by a medicinal fungus Cordyceps sinensis Cs-HK1. Its carbohydrate moiety was mainly composed of glucose and mannose at 3.2:1.0 mole ratio, indicating an O-linked glycopeptide. The peptide chain contained relatively high mole ratios of aspartic acid, glutamic acid and glycine (3.3-3.5 relative to arginine) but relatively low ratios of tyrosine and histidine. The peptide chain sequence analyzed after trypsin digestion by LC-MS was KNGIFQFGEDCAAGSISHELGGFREFREFLKQAGLE. Cs-GP1 exhibited remarkable antioxidant capacity with a Trolox equivalent antioxidant capacity of 1183.8 mu mol/g and a ferric reducing ability of 611.1 mu mol Fe(II)/g, and significant protective effect against H2O2-induced PC12 cell injury at a minimum dose of 10 mu g/mL. This is the first report on the structure and bioactivity of an extracellular glycopeptide from the Cordyceps species. | Keywords: | Cordyceps sinensis Glycopeptide Structure Antioxidant Cell protection |
Publisher: | Molecular Diversity Preservation International (MDPI) | Journal: | International journal of molecular sciences | ISSN: | 1661-6596 | EISSN: | 1422-0067 | DOI: | 10.3390/ijms151017318 | Rights: | © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). The following publication Wu, J. Y., Chen, X., & Siu, K. C. (2014). Isolation and structure characterization of an antioxidative glycopeptide from mycelial culture broth of a medicinal fungus. International Journal of Molecular Sciences, 15(10), (Suppl. ), 17318-17332 is available athttps://dx.doi.org/10.3390/ijms151017318 |
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