Isolation and structure characterization of an antioxidative glycopeptide from mycelial culture broth of a medicinal fungus

Abstract

A novel glycopeptide (Cs-GP1) with an average molecular weight (Mw) of 6.0 kDa was isolated and purified by column chromatography from the lower Mw fraction of exopolysaccharide (EPS) produced by a medicinal fungus Cordyceps sinensis Cs-HK1. Its carbohydrate moiety was mainly composed of glucose and mannose at 3.2:1.0 mole ratio, indicating an O-linked glycopeptide. The peptide chain contained relatively high mole ratios of aspartic acid, glutamic acid and glycine (3.3-3.5 relative to arginine) but relatively low ratios of tyrosine and histidine. The peptide chain sequence analyzed after trypsin digestion by LC-MS was KNGIFQFGEDCAAGSISHELGGFREFREFLKQAGLE. Cs-GP1 exhibited remarkable antioxidant capacity with a Trolox equivalent antioxidant capacity of 1183.8 mu mol/g and a ferric reducing ability of 611.1 mu mol Fe(II)/g, and significant protective effect against H2O2-induced PC12 cell injury at a minimum dose of 10 mu g/mL. This is the first report on the structure and bioactivity of an extracellular glycopeptide from the Cordyceps species.