Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/32570
Title: Differential ERα-mediated rapid estrogenic actions of ginsenoside Rg1 and estren in human breast cancer MCF-7 cells
Authors: Gao, QG
Chan, HY
Man, CWY
Wong, MS 
Keywords: 17β-Estradiol
Estren
Estrogen receptor
Ginsenoside Rg1
MCF-7 cells
Issue Date: 2014
Source: Journal of steroid biochemistry and molecular biology, 2014, v. 141, p. 104-112 How to cite?
Journal: Journal of Steroid Biochemistry and Molecular Biology 
Abstract: Recent studies indicated that both estren and Rg1 appear to be able to activate mitogen-activated protein kinase (MAPK) pathway in estrogen responsive cells. Rg1 could lead to MAPK activation through ligand-independent activation of estrogen receptor (ER), while estren could activate the Src-MAPK pathway in an ERE-independent manner. Thus, it is important to understand the mechanistic insights on the difference in transcriptional activation between estren and Rg1. The present study also addressed the differential abilities of Rg1 and estren in terms of the ability to activate ER and the ability to induce ER translocation in MCF-7 cells. Our data indicated that Rg1 could increase pS2 gene expression, and could recruit the co-activator steroid receptor co-activator-1 (SRC-1) to the pS2 promoter. Rg1 could also induce ERα nuclear translocation as well as ERα phosphorylation at Ser118 principally in the cytoplasm in MCF-7 cells. We deduced that estren induced ERE-dependent transcriptional activity and activated ERα at Ser118 occurred in the nucleus of MCF-7 cells. However, it was found to decrease pS2 gene expression and failed to induce the recruitment of SRC-1 to the pS2 promoter in MCF-7 cells. Our results suggest that the abilities of Rg1 and estren to regulate pS2 gene expression, to recruit co-activators as well as to induce sub-cellular distribution of ERα are dramatically different.
URI: http://hdl.handle.net/10397/32570
ISSN: 0960-0760
DOI: 10.1016/j.jsbmb.2014.01.014
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