Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/91991
DC Field | Value | Language |
---|---|---|
dc.contributor | Department of Applied Biology and Chemical Technology | en_US |
dc.contributor | Chinese Mainland Affairs Office | en_US |
dc.contributor | Research Institute for Future Food | - |
dc.contributor | Department of Applied Biology and Chemical Technology | - |
dc.contributor | Chinese Mainland Affairs Office | - |
dc.creator | Huang, L | en_US |
dc.creator | So, PK | en_US |
dc.creator | Chen, YW | en_US |
dc.creator | Leung, YC | en_US |
dc.creator | Yao, ZP | en_US |
dc.date.accessioned | 2022-02-07T07:04:50Z | - |
dc.date.available | 2022-02-07T07:04:50Z | - |
dc.identifier.issn | 0021-9258 | en_US |
dc.identifier.uri | http://hdl.handle.net/10397/91991 | - |
dc.language.iso | en | en_US |
dc.publisher | American Society for Biochemistry and Molecular Biology | en_US |
dc.rights | © 2021 THE AUTHORS. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology. This is an open access article under the CCBY license (http://creativecommons.org/licenses/by/4.0/). | en_US |
dc.rights | The following publication Huang, L., So, P. K., Chen, Y. W., Leung, Y. C., & Yao, Z. P. (2021). Interdomain flexibility and interfacial integrity of β-lactamase inhibitory protein (BLIP) modulate its binding to class A β-lactamases. Journal of Biological Chemistry, 297(2) is available at https://doi.org/10.1016/j.jbc.2021.100980 | en_US |
dc.subject | β-lactamases | en_US |
dc.subject | β-lactamase inhibitory protein (BLIP) | en_US |
dc.subject | Interdomain flexibility | en_US |
dc.subject | Interfacial integrity | en_US |
dc.subject | Hydrogen deuterium exchange mass spectrometry | en_US |
dc.subject | Molecular dynamics simulation | en_US |
dc.title | Interdomain flexibility and interfacial integrity of β-lactamase inhibitory protein (BLIP) modulate its binding to class A β-lactamases | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.volume | 297 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.doi | 10.1016/j.jbc.2021.100980 | en_US |
dcterms.abstract | β-Lactamase inhibitory protein (BLIP) consists of a tandem repeat of αβ domains conjugated by an interdomain loop and can effectively bind and inactivate class A β-lactamases, which are responsible for resistance of bacteria to β-lactam antibiotics. The varied ability of BLIP to bind different β-lactamases and the structural determinants for significant enhancement of BLIP variants with a point mutation are poorly understood. Here, we investigated the conformational dynamics of BLIP upon binding to three clinically prevalent class A β-lactamases (TEM1, SHV1, and PC1) with dissociation constants between subnanomolar and micromolar. Hydrogen deuterium exchange mass spectrometry revealed that the flexibility of the interdomain region was significantly suppressed upon strong binding to TEM1, but was not significantly changed upon weak binding to SHV1 or PC1. E73M and K74G mutations in the interdomain region improved binding affinity toward SHV1 and PC1, respectively, showing significantly increased flexibility of the interdomain region compared to the wild-type and favorable conformational changes upon binding. In contrast, more rigidity of the interfacial loop 135-145 was observed in these BLIP mutants in both free and bound states. Consistently, molecular dynamics simulations of BLIP exhibited drastic changes in the flexibility of the loop 135-145 in all complexes. Our results indicated for the first time that higher flexibility of the interdomain linker, as well as more rigidity of the interfacial loop 135-145, could be desirable determinants for enhancing inhibition of BLIP to class A β-lactamases. Together, these findings provide unique insights into the design of enhanced inhibitors. | en_US |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | Journal of biological chemistry, Aug. 2021, v. 297, no. 2, 100980 | en_US |
dcterms.isPartOf | Journal of biological chemistry | en_US |
dcterms.issued | 2021-08 | - |
dc.identifier.scopus | 2-s2.0-85112824664 | - |
dc.identifier.pmid | 34302811 | - |
dc.identifier.eissn | 1083-351X | en_US |
dc.identifier.artn | 100980 | en_US |
dc.description.validate | 202202 bcvc | en_US |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | RGC-B1-134b, OA_Scopus/WOS | - |
dc.description.fundingSource | RGC | en_US |
dc.description.fundingSource | Others | en_US |
dc.description.fundingText | This work was supported by Hong Kong Research Grants Council (Grant Nos. 153040/15P, 153348/16P, 153041/17P, 15304020, R5013-19F, C5031-14E, C4002-17G, and R4005-18), Natural Science Foundation of China (Grant No. 81874306), and China Resources Life Sciences Group Limited. | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
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1-s2.0-S0021925821007821-main.pdf | 2.95 MB | Adobe PDF | View/Open |
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