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Title: Transcriptome profiling of mouse corpus callosum after cerebral hypoperfusion
Authors: Takase, H
Hamanaka, G
Ohtomo, R
Ishikawa, H
Chung, KK
Mandeville, ET
Lok, J
Fornage, M
Herrup, K
Tse, KH 
Lo, EH
Arai, K
Issue Date: Jun-2021
Source: Frontiers in cell and developmental biology, June 2021, v. 9, 685261
Abstract: White matter damage caused by cerebral hypoperfusion is a major hallmark of subcortical ischemic vascular dementia (SIVD), which is the most common subtype of vascular cognitive impairment and dementia (VCID) syndrome. In an aging society, the number of SIVD patients is expected to increase; however, effective therapies have yet to be developed. To understand the pathological mechanisms, we analyzed the profiles of the cells of the corpus callosum after cerebral hypoperfusion in a preclinical SIVD model. We prepared cerebral hypoperfused mice by subjecting 2-month old male C57BL/6J mice to bilateral carotid artery stenosis (BCAS) operation. BCAS-hypoperfusion mice exhibited cognitive deficits at 4 weeks after cerebral hypoperfusion, assessed by novel object recognition test. RNA samples from the corpus callosum region of sham- or BCAS-operated mice were then processed using RNA sequencing. A gene set enrichment analysis using differentially expressed genes between sham and BCAS-operated mice showed activation of oligodendrogenesis pathways along with angiogenic responses. This database of transcriptomic profiles of BCAS-hypoperfusion mice will be useful for future studies to find a therapeutic target for SIVD.
Keywords: Cerebral hypoperfusion
Corpus callosum
White matter
Publisher: Frontiers Research Foundation
Journal: Frontiers in cell and developmental biology 
EISSN: 2296-634X
DOI: 10.3389/fcell.2021.685261
Rights: Copyright © 2021 Takase, Hamanaka, Ohtomo, Ishikawa, Chung, Mandeville, Lok, Fornage, Herrup, Tse, Lo and Arai.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) ( The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
The following publication Takase H, Hamanaka G, Ohtomo R, Ishikawa H, Chung KK, Mandeville ET, Lok J, Fornage M, Herrup K, Tse K-H, Lo EH and Arai K (2021) Transcriptome Profiling of Mouse Corpus Callosum After Cerebral Hypoperfusion. Front. Cell Dev. Biol. 9:685261 is available at
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