Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/91682
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dc.contributorCollege of Professional and Continuing Education-
dc.creatorWang, QG-
dc.creatorCheng, BCY-
dc.creatorHe, YZ-
dc.creatorLi, LJ-
dc.creatorLing, Y-
dc.creatorLuo, G-
dc.creatorWang, L-
dc.creatorLiang, S-
dc.creatorZhang, Y-
dc.date.accessioned2021-11-24T06:07:42Z-
dc.date.available2021-11-24T06:07:42Z-
dc.identifier.issn1792-0981-
dc.identifier.urihttp://hdl.handle.net/10397/91682-
dc.language.isoenen_US
dc.publisherSpandidos Publicationsen_US
dc.rights© Wang et al.en_US
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License (https://creativecommons.org/licenses/by-nc-nd/4.0/).en_US
dc.rightsThe following publication Wang, Q. G., Cheng, B. C. Y., He, Y. Z., Li, L. J., Ling, Y., Luo, G., ... & Zhang, Y. (2021). miR‑320a in serum exosomes promotes myocardial fibroblast proliferation via regulating the PIK3CA/Akt/mTOR signaling pathway in HEH2 cells. Experimental and Therapeutic Medicine, 22(2), 1-14 is available at https://doi.org/10.3892/etm.2021.10305en_US
dc.subjectCHFen_US
dc.subjectExosomeen_US
dc.subjectHEH2 cellsen_US
dc.subjectMyocardial fibrosisen_US
dc.subjectPIK3CAen_US
dc.subjectAkten_US
dc.subjectMTOR signaling pathwayen_US
dc.titlemiR-320a in serum exosomes promotes myocardial fibroblast proliferation via regulating the PIK3CA/Akt/mTOR signaling pathway in HEH2 cellsen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.volume22-
dc.identifier.issue2-
dc.identifier.doi10.3892/etm.2021.10305-
dcterms.abstractMicroRNAs (miRNAs/miRs) serve an important role in the pathogenesis of chronic heart failure (CHF). A number of reports have illustrated the regulatory effect of serum exosomal miRNA on myocardial fibrosis. The present study aimed to investigate the expression of miR-320a in serum exosomes, as well as the effect of miR-320a on myocardial fibroblast proliferation. Serum exosome samples from 10 patients with CHF and 5 healthy volunteers were obtained and characterized. mRNA and protein expression levels were measured via reverse transcription-quantitative PCR and western blotting, respectively. The content of soluble growth stimulation expressed gene 2 (sST2) was determined via ELISA. HEH2 cell viability and apoptosis were detected by performing MTT assays and flow cytometry, respectively. The results demonstrated that serum miR-320a expression levels and sST2 content were significantly increased in patients with CHF compared with healthy controls, and the expression of serum miR-320a was significantly correlated with clinical CHF indexes. miR-320a expression levels were significantly increased in exosomes isolated from patients with CHF compared with those isolated from healthy controls. Phosphoinositide-3-kinase catalytic alpha polypeptide gene (PIK3CA) expression levels and sST2 content were increased in HEH2 cells following transfection with miR-320a mimics compared with NC-mimic, whereas miR-320a inhibitor displayed contrasting effects by reduced the cell viability and apoptosis in myocardial fibroblasts compared with the NC-inhibitor group. The protein expression levels of collagen I, collagen III, alpha-smooth muscle actin, phosphorylated (p)-mTOR (ser 2448)/mTOR, p-Akt (ser 473)/Akt, p-Akt (thr 308)/Akt and PIK3CA were significantly increased in miR-320a mimic-transfected HEH2 cells compared with the NC-mimics groups. By contrast, miR-320a inhibitor notably downregulated the expression levels of these proteins compared with the NC-inhibitor group. Collectively, the results of the present study demonstrated that miR-320a promoted myocardial fibroblast proliferation via regulating the PIK3CA/Akt/mTOR signaling pathway in HEH2 cells, suggesting that serum exosomal miR-320a may serve as a potential biomarker for the diagnosis of CHF.-
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationExperimental and therapeutic medicine, Aug. 2021, v. 22, no. 2, 873-
dcterms.isPartOfExperimental and therapeutic medicine-
dcterms.issued2021-08-
dc.identifier.isiWOS:000667605400001-
dc.identifier.pmid34194551-
dc.identifier.eissn1792-1015-
dc.identifier.artn873-
dc.description.validate202111 bchy-
dc.description.oaVersion of Recorden_US
dc.identifier.FolderNumberOA_Scopus/WOSen_US
dc.description.pubStatusPublisheden_US
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