Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/91675
| Title: | Molecular characterization and expression of cd96 in nile tilapia (oreochromis niloticus) in response to different pathogens stimulus | Authors: | Xie, CX Wang, ZW Li, Y Wu, F Xia, HL Lu, YS Tang, JF Jian, JC Kwok, KWH |
Issue Date: | Jul-2021 | Source: | Aquaculture reports, July 2021, v. 20, 100705 | Abstract: | CD96 is one of the immune superfamily members and it is identified as a T cell-specific receptor. In this study, we cloned a CD96 from Nile tilapia (Oreochromis niloticus, named OnCD96). The open reading frame of OnCD96was 1371 bp, encoding a protein of 456 amino acids. Sequence alignment analysis indicated that OnCD96 contained two Ig-like domains in entodomain, proline-rich motif, and ITIM motif in the cytoplasm. Subcellular localization studies showed that OnCD96 was distributed mainly in the cytoplasm and on the cytoplasmic membrane. In healthy tilapia, OnCD96 was distributed in all the tested tissues and relatively higher in the liver. After Streptococcus agalactiae infection, the expression of OnCD96 in the thymus, brain, and spleen reached its peak at 72 h, 48 h, and 24 h, respectively. After stimulation with poly I:C, the expression of OnCD96 in thymus and spleen reached its peak at 6 h and 24 h respectively. While, the expression in the brain had double peaks, which were at 24 h and 96 h. After stimulation with Keyhole limpet hemocyanin (KLH), a classical T cell-dependent antigen, the expression of OnCD96 was significantly up-regulated in blood and head kidney. Moreover, compared with the first challenge, OnCD96 was up-regulated earlier after the second challenge. Yeast two-hybrid assay indicated that there might be interaction between OnCD96 and OnNecl5. These results suggested that OnCD96 plays an important role during pathogens infection, and the interaction between CD96 and Necl5 is conserved in Nile tilapia. | Keywords: | CD96 Necl5 Nile tilapia T cell-dependent response Yeast two-hybrid |
Publisher: | Elsevier BV | Journal: | Aquaculture reports | EISSN: | 2352-5134 | DOI: | 10.1016/j.aqrep.2021.100705 | Rights: | © 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/) The following publication Xie, C., Wang, Z., Li, Y., Wu, F., Xia, H., Lu, Y., ... & Kwok, K. W. (2021). Molecular characterization and expression of CD96 in Nile tilapia (Oreochromis niloticus) in response to different pathogens stimulus. Aquaculture Reports, 20, 100705 is available at https://doi.org/10.1016/j.aqrep.2021.100705 |
| Appears in Collections: | Journal/Magazine Article |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Xie_Molecular_characterization_expression.pdf | 5.98 MB | Adobe PDF | View/Open |
Access
View full-text via PolyU eLinks 
Page views
38
Last Week
0
0
Last month
Citations as of Jun 4, 2023
Downloads
18
Citations as of Jun 4, 2023
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.