Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/91655
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dc.contributorCollege of Professional and Continuing Educationen_US
dc.creatorYan, LSen_US
dc.creatorWang, Len_US
dc.creatorCheng, BCYen_US
dc.creatorDing, Yen_US
dc.creatorKong, Jen_US
dc.creatorWang, QGen_US
dc.creatorFu, XQen_US
dc.creatorZhang, SFen_US
dc.creatorLuo, Gen_US
dc.creatorZhang, Yen_US
dc.date.accessioned2021-11-24T06:07:19Z-
dc.date.available2021-11-24T06:07:19Z-
dc.identifier.issn2221-1691en_US
dc.identifier.urihttp://hdl.handle.net/10397/91655-
dc.language.isoenen_US
dc.publisherWolters Kluwer - Medknow Publications and Media Pvt. Ltd.en_US
dc.rights©2021 Asian Pacific Journal of Tropical Biomedicine Produced by Wolters KluwerMedknow. All rights reserved.en_US
dc.rightsThis is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-Non Commercial-ShareAlike 4.0 License (https://creativecommons.org/licenses/by-nc-sa/4.0/), which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.en_US
dc.rightsThe following publication Yan LS, Wang L, Cheng BC, Ding Y, Kong J, Wang QG, et al. Total flavonoids from Saussurea involucrata attenuate inflammation in lipopolysaccharide-stimulated RAW264.7 macrophages via modulating p65, c-Jun, and IRF3 signaling pathways. Asian Pac J Trop Biomed 2021; 11(6): 273-284 is available at https://www.doi.org/10.4103/2221-1691.314053en_US
dc.subjectSaussurea involucrataen_US
dc.subjectCytokinesen_US
dc.subjectProinflammatoryen_US
dc.subjectMacrophagesen_US
dc.subjectFlavonoidsen_US
dc.titleTotal flavonoids from Saussurea involucrata attenuate inflammation in lipopolysaccharide-stimulated RAW264.7 macrophages via modulating p65, c-Jun, and IRF3 signaling pathwaysen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage273en_US
dc.identifier.epage284en_US
dc.identifier.volume11en_US
dc.identifier.issue6en_US
dc.identifier.doi10.4103/2221-1691.314053en_US
dcterms.abstractObjective: To investigate the anti-inflammatory effects of the total flavonoids from Saussurea involucrata on lipopolysaccharides (LPS)-stimulated murine RAW264.7 macrophages and explore its underlying mechanism of action.en_US
dcterms.abstractMethods: Total flavonoids from Saussurea involucrata were extracted using chromatographic column method. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The production of nitric oxide was detected by Griess assay and the release of cytokines (IL-10 and TNF-alpha) and chemokines (MCP-1, MIP-1a, and CCL5/RANTES) was determined by ELISA to evaluate the anti-inflammatory activity of total flavonoids from Saussurea involucrata. Moreover, nuclear translocation of p65, c-Jun, and IRF3 was detected by immunofluorescence microscopy and Western blotting analysis was performed to determine the expression of related proteins.en_US
dcterms.abstractResults: Total flavonoids extracted from Saussurea involucrata were 751.5 mg/g and the content of rutin was 506.5 mg/g. The production of inflammatory mediators including nitric oxide, cytokines, and chemokines was effectively inhibited by total flavonoids from Saussurea involucrata. Meanwhile, total flavonoids also suppressed the nuclear translocation of p65, c-Jun, and IRF3 in LPS-stimulated RAW264.7 cells. The LPS-induced expression of iNOS and COX-2 was remarkably reduced by treatment with total flavonoids from Saussurea involucrata. Moreover, total flavonoids decreased the expression levels of p-IKKa/beta, p-TBK1, p-p38, p-ERK, p-JNK, p-p65, p-c-Jun, and p-IRF3 in LPS-exposed RAW264.7 macrophages.en_US
dcterms.abstractConclusions: Total flavonoids from Saussurea involucrata potentially inhibit the secretion of pro-inflammatory mediators, which may be related to inhibition of p65, c-Jun, and IRF3 signaling pathways in LPS-stimulated RAW264.7 cells.en_US
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationAsian pacific journal of tropical biomedicine, 2021, v. 11, no. 6, p. 273-284en_US
dcterms.bibliographicCitationAsian pacific journal of tropical biomedicine, 2021, v. 11, no. 6, p. 273-284-
dcterms.isPartOfAsian pacific journal of tropical biomedicineen_US
dcterms.issued2021-
dc.identifier.isiWOS:000651319800005-
dc.identifier.eissn2588-9222en_US
dc.description.validate202111 bchyen_US
dc.description.oaVersion of Recorden_US
dc.identifier.FolderNumberOA_Scopus/WOS-
dc.description.pubStatusPublisheden_US
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