Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/91325
PIRA download icon_1.1View/Download Full Text
DC FieldValueLanguage
dc.contributorDepartment of Biomedical Engineering-
dc.creatorYang, B-
dc.creatorWei, K-
dc.creatorLoebel, C-
dc.creatorZhang, K-
dc.creatorFeng, Q-
dc.creatorLi, R-
dc.creatorWong, SHD-
dc.creatorXu, X-
dc.creatorLau, C-
dc.creatorChen, X-
dc.creatorZhao, P-
dc.creatorYin, C-
dc.creatorBurdick, JA-
dc.creatorWang, Y-
dc.creatorBian, L-
dc.date.accessioned2021-11-03T06:52:41Z-
dc.date.available2021-11-03T06:52:41Z-
dc.identifier.urihttp://hdl.handle.net/10397/91325-
dc.language.isoenen_US
dc.publisherNature Publishing Groupen_US
dc.rights© The Author(s) 2021en_US
dc.rightsOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.en_US
dc.rightsThe following publication Yang, B., Wei, K., Loebel, C. et al. Enhanced mechanosensing of cells in synthetic 3D matrix with controlled biophysical dynamics. Nat Commun 12, 3514 (2021) is available at https://doi.org/10.1038/s41467-021-23120-0en_US
dc.titleEnhanced mechanosensing of cells in synthetic 3D matrix with controlled biophysical dynamicsen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.volume12-
dc.identifier.doi10.1038/s41467-021-23120-0-
dcterms.abstract3D culture of cells in designer biomaterial matrices provides a biomimetic cellular microenvironment and can yield critical insights into cellular behaviours not available from conventional 2D cultures. Hydrogels with dynamic properties, achieved by incorporating either degradable structural components or reversible dynamic crosslinks, enable efficient cell adaptation of the matrix and support associated cellular functions. Herein we demonstrate that given similar equilibrium binding constants, hydrogels containing dynamic crosslinks with a large dissociation rate constant enable cell force-induced network reorganization, which results in rapid stellate spreading, assembly, mechanosensing, and differentiation of encapsulated stem cells when compared to similar hydrogels containing dynamic crosslinks with a low dissociation rate constant. Furthermore, the static and precise conjugation of cell adhesive ligands to the hydrogel subnetwork connected by such fast-dissociating crosslinks is also required for ultra-rapid stellate spreading (within 18 h post-encapsulation) and enhanced mechanosensing of stem cells in 3D. This work reveals the correlation between microscopic cell behaviours and the molecular level binding kinetics in hydrogel networks. Our findings provide valuable guidance to the design and evaluation of supramolecular biomaterials with cell-adaptable properties for studying cells in 3D cultures.-
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationNature communications, 2021, v. 12, 3514-
dcterms.isPartOfNature communications-
dcterms.issued2021-
dc.identifier.scopus2-s2.0-85107590087-
dc.identifier.pmid34112772-
dc.identifier.eissn2041-1723-
dc.identifier.artn3514-
dc.description.validate202110 bcvc-
dc.description.oaVersion of Recorden_US
dc.identifier.FolderNumberOA_Scopus/WOSen_US
dc.description.pubStatusPublisheden_US
Appears in Collections:Journal/Magazine Article
Files in This Item:
File Description SizeFormat 
s41467-021-23120-0.pdf3.49 MBAdobe PDFView/Open
Open Access Information
Status open access
File Version Version of Record
Access
View full-text via PolyU eLinks SFX Query
Show simple item record

Page views

12
Citations as of Jun 26, 2022

Downloads

2
Citations as of Jun 26, 2022

SCOPUSTM   
Citations

26
Citations as of Jun 30, 2022

WEB OF SCIENCETM
Citations

21
Citations as of Jun 30, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.