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http://hdl.handle.net/10397/90713
| Title: | Functionalized gold nanoparticles for point-of-care nucleic acid detection | Authors: | Lee, TMH | Issue Date: | 2015 | Source: | World Congress on Medical Physics & Biomedical Engineering, 7-12 June 2015, Toronto, Canada, p.1 (Abstract) | Abstract: | Gold nanoparticles (AuNPs) have been extensively investigated for colorimetric detection of nucleic acid. This is enabled by the unique interparticle distance-dependent optical property of AuNPs. The solution color for monodispersed particles appears red (for widely used 13 nm AuNPs) but turns purple upon aggregation due to a red-shift in the surface plasmon resonance absorption band. Until now, all the reported platforms are not practical for point-of-care testing. To address this, our group developed a new platform by incorporating 11-mercaptoundecanoic acid-modified AuNPs (MUA–AuNPs) into loop-mediated isothermal amplification (LAMP). When added into the LAMP reaction mixture, MUA–AuNPs aggregated as a result of ion-templated chelation between the carboxyl groups and magnesium ion (Mg2+, which plays an indispensable role in LAMP reaction as an enzyme cofactor). The solution color changed from red to purple. In the presence of a specific target DNA sequence, the LAMP reaction occurred and pyrophosphate ion (P2O74−) was generated as a reaction by-product. The chelated Mg2+ was then extracted by P2O74−, leading to the deaggregation/redispersion of the MUA–AuNPs and the solution color turned red. This new platform possesses all the ideal features for point-of-care testing, including simple preparation and operation, low cost, high sensitivity, and worry-free carryover contamination control. | Rights: | Posted with permission of the author. |
| Appears in Collections: | Conference Paper |
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|---|---|---|---|---|
| a0842-n04_1731.pdf | 935.39 kB | Adobe PDF | View/Open |
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