Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/89713
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dc.contributorSchool of Optometry-
dc.creatorBian, Jen_US
dc.creatorSze, YHen_US
dc.creatorTse, DYYen_US
dc.creatorTo, CHen_US
dc.creatorMcFadden, SAen_US
dc.creatorLam, CSYen_US
dc.creatorLi, KKen_US
dc.creatorLam, TCen_US
dc.date.accessioned2021-05-05T04:56:58Z-
dc.date.available2021-05-05T04:56:58Z-
dc.identifier.issn1661-6596en_US
dc.identifier.urihttp://hdl.handle.net/10397/89713-
dc.language.isoenen_US
dc.publisherMolecular Diversity Preservation International (MDPI)en_US
dc.rightsCopyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).en_US
dc.subjectSWATH-MSen_US
dc.subjectProteomicsen_US
dc.subjectMyopiaen_US
dc.subjectRetinaen_US
dc.subjectGuinea pigsen_US
dc.subjectLipid metabolismen_US
dc.titleSWATH based quantitative proteomics reveals significant lipid metabolism in early myopic guinea pig retinaen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.volume22en_US
dc.identifier.issue9en_US
dc.identifier.doi10.3390/ijms22094721en_US
dcterms.abstractMost of the previous myopic animal studies employed a single-candidate approach and lower resolution proteomics approaches that were difficult to detect minor changes, and generated limited systems-wide biological information. Hence, a complete picture of molecular events in the retina involving myopic development is lacking. Here, to investigate comprehensive retinal protein alternations and underlying molecular events in the early myopic stage, we performed a data-independent Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH) based proteomic analysis coupled with different bioinformatics tools in pigmented guinea pigs after 4-day lens-induced myopia (LIM). Myopic eyes compared to untreated contralateral control eyes caused significant changes in refractive error and choroid thickness (p < 0.05, n = 5). Relative elongation of axial length and the vitreous chamber depth were also observed. Using pooled samples from all individuals (n = 10) to build a species-specific retinal ion library for SWATH analysis, 3202 non-redundant proteins (with 24,616 peptides) were identified at 1% global FDR. For quantitative analysis, the 10 individual retinal samples (5 pairs) were analyzed using a high resolution Triple-TOF 6600 mass spectrometry (MS) with technical replicates. In total, 37 up-regulated and 21 down-regulated proteins were found significantly changed after LIM treatment (log2 ratio (T/C) > 0.26 or < −0.26; p ≤ 0.05). Data are accepted via ProteomeXchange with identifier PXD025003. Through Ingenuity Pathways Analysis (IPA), “lipid metabolism” was found as the top function associated with the differentially expressed proteins. Based on the protein abundance and peptide sequences, expression patterns of two regulated proteins (SLC6A6 and PTGES2) identified in this pathway were further successfully validated with high confidence (p < 0.05) using a novel Multiple Reaction Monitoring (MRM) assay on a QTRAP 6500+ MS. In summary, through an integrated discovery and targeted proteomic approach, this study serves as the first report to detect and confirm novel retinal protein changes and significant biological functions in the early LIM mammalian guinea pigs. The study provides new workflow and insights for further research to myopia control.-
dcterms.accessRightsopen access-
dcterms.bibliographicCitationInternational journal of molecular sciences, 1 May 2021, v. 22, no. 9, 4721en_US
dcterms.isPartOfInternational journal of molecular sciencesen_US
dcterms.issued2021-05-
dc.identifier.scopus2-s2.0-85105481130-
dc.identifier.eissn1422-0067en_US
dc.identifier.artn4721en_US
dc.description.validate202105 bcwh-
dc.description.oaVersion of Record-
dc.identifier.FolderNumbera0880-n01-
dc.description.fundingSourceRGC-
dc.description.fundingSourceOthers-
dc.description.fundingTextFunding acknowledgements: This work was supported by PolyU PhD Studentship (RTX2); RGC General Research Fund (15104819, 15102015/15M); Henry G. Leong Endowed Professorship in Elderly Vision Health; Dean’s Reserve (ZVN2); Shenzhen Science and Technology Innovation Commission (JCYJ20180507183409601); Australian Government Endeavour Cheung Kong Research Fellowship G0189472 (DYT, SMF); Australian DIIRSE International Science Linkage CG120160 (SMF).-
dc.description.pubStatusPublished-
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