Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/89213
Title: | Longitudinal outcomes of circumlimbal suture model-induced chronic ocular hypertension in Sprague-Dawley albino rats | Authors: | Lakshmanan, Y Wong, FSY Zuo, B Bui, BV Chan, HHL |
Issue Date: | Dec-2020 | Source: | Graefe's archive for clinical and experimental ophthalmology, Dec 2020, v. 258, no. 12, p. 2715-2728 | Abstract: | Purpose: To characterise longitudinal structural and functional changes in albino Sprague-Dawley rats following circumlimbal suture ocular hypertension (OHT) induction. Methods: Ten-week-old rats (n = 24) underwent suture implantation around the limbal region in both eyes. On the next day, the suture was removed from one eye (control eyes) and left intact in the other eye (OHT eyes) of each animal. Intraocular pressure (IOP) was monitored weekly twice for the next 15 weeks. Optical coherence tomography (OCT) and electroretinogram (ERG) were measured at baseline and weeks 4, 8, 12, and 15, and eyes were then collected for histological assessment. Results: Sutured eyes (n = 12) developed IOP elevation of ~ 50% in the first 2 weeks that was sustained at ~ 25% above the control eye up to week 15 (p = 0.001). Animals with insufficient IOP elevation (n = 6), corneal changes (n = 3), and attrition (n = 3) were excluded from the analysis. OHT eyes developed significant retinal nerve fibre layer (RNFL) thinning (week 4: − 19 ± 14%, p = 0.10; week 8: − 17 ± 12%, p = 0.04; week 12: − 16 ± 10%, p = 0.04, relative to baseline) and reduction in retinal ganglion cell (RGC) density (− 32 ± 26%, p = 0.02). At week 15, both inner (9 ± 7%, p = 0.01) and outer retinal layer thicknesses (6.0 ± 5%, p = 0.001) showed a mild increase in thicknesses. The positive scotopic threshold response (− 28 ± 25%, p = 0.04) and a-wave were significantly reduced at week 12 (− 35 ± 21%; p = 0.04), whereas b-wave was not significantly affected (week 12: − 18 ± 27%, p = 0.24). Conclusion: The circumlimbal suture model produced a chronic, moderate IOP elevation in an albino strain that led to RNFL thinning and reduced RGC density along with the reductions in ganglion and photoreceptoral cell functions. There was a small thickening in both outer and inner retinal layers. |
Keywords: | Albino rat Animal model Circumlimbal suture Glaucoma Intraocular pressure Ocular hypertension |
Publisher: | Springer | Journal: | Graefe's archive for clinical and experimental ophthalmology | ISSN: | 0721-832X | EISSN: | 1435-702X | DOI: | 10.1007/s00417-020-04820-7 | Rights: | © Springer-Verlag GmbH Germany, part of Springer Nature 2020 This is a post-peer-review, pre-copyedit version of an article published in Graefe's Archive for Clinical and Experimental Ophthalmology. The final authenticated version is available online at: https://doi.org/https://doi.org/10.1007/s00417-020-04820-7. |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
a0764-n15_1619_Main_Text.pdf | Pre-Published version | 2.19 MB | Adobe PDF | View/Open |
a0764-n15_1619_Supplementary_Materials.pdf | Pre-Published version | 1.29 MB | Adobe PDF | View/Open |
Page views
43
Last Week
1
1
Last month
Citations as of Jun 4, 2023
Downloads
50
Citations as of Jun 4, 2023
SCOPUSTM
Citations
4
Citations as of Jun 1, 2023
WEB OF SCIENCETM
Citations
3
Citations as of Jun 1, 2023

Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.