Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/88988
DC Field | Value | Language |
---|---|---|
dc.contributor | Department of Biomedical Engineering | en_US |
dc.creator | Chen, Z | en_US |
dc.creator | Zhang, Q | en_US |
dc.creator | Li, H | en_US |
dc.creator | Wei, Q | en_US |
dc.creator | Zhao, X | en_US |
dc.creator | Chen, F | en_US |
dc.date.accessioned | 2021-01-15T07:14:38Z | - |
dc.date.available | 2021-01-15T07:14:38Z | - |
dc.identifier.uri | http://hdl.handle.net/10397/88988 | - |
dc.language.iso | en | en_US |
dc.publisher | Ke Ai Publishing Communications Ltd. | en_US |
dc.rights | © 2020 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/). | en_US |
dc.rights | The following publication Chen, Z., Zhang, Q., Li, H., Wei, Q., Zhao, X., & Chen, F. (2020). Elastin-like polypeptide modified silk fibroin porous scaffold promotes osteochondral repair. Bioactive materials, 6(3), 589-601, is available at https://doi.org/10.1016/j.bioactmat.2020.09.003 | en_US |
dc.subject | Bone repair | en_US |
dc.subject | Cartilage repair | en_US |
dc.subject | Elastin-Like polypeptide | en_US |
dc.subject | Silk fiber | en_US |
dc.title | Elastin-like polypeptide modified silk fibroin porous scaffold promotes osteochondral repair | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.spage | 589 | en_US |
dc.identifier.epage | 601 | en_US |
dc.identifier.volume | 6 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.doi | 10.1016/j.bioactmat.2020.09.003 | en_US |
dcterms.abstract | Silk fibroin (SF) is considered biocompatible and biodegradable for osteochondral repair. However, it lacks a bioactive domain for cell adhesion, proliferation and differentiation, limiting its therapeutic efficacy. To revamp SF as a biomimicking and bioactive microenvironment to regulate cell behaviours, we engineered an elastin-like polypeptide (ELP, Val-Pro-Gly-Xaa-Gly) to modify SF fibers via simple and green dehydrothermal (DHT) treatment. Our results demonstrated that the ELP successfully bound to SF, and the scaffold was reinforced by the fusion of the silk fiber intersections with ELP (S-ELP-DHT) via the DHT treatment. Both bone mesenchymal stem cells (BMSCs) and chondrocytes exhibited improved spreading and proliferation on the S-ELP-DHT scaffolds. The ex vivo and in vivo experiments further demonstrated enhanced mature bone and cartilage tissue formation using the S-ELP-DHT scaffolds compared to the naked SF scaffolds. These results indicated that a recombinant ELP-modified silk scaffold can mimic three-dimensional (3D) cell microenvironment, and improve bone and cartilage regeneration. We envision that our scaffolds have huge clinical potential for osteochondral repair. | en_US |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | Bioactive materials, Mar. 2021, v. 6, no. 3, p. 589-601 | en_US |
dcterms.isPartOf | Bioactive materials | en_US |
dcterms.issued | 2021-03 | - |
dc.identifier.scopus | 2-s2.0-85091218640 | - |
dc.identifier.eissn | 2452-199X | en_US |
dc.description.validate | 202101 bcrc | en_US |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | a0596-n07, OA_Scopus/WOS | en_US |
dc.identifier.SubFormID | 425 | - |
dc.description.fundingSource | Self-funded | en_US |
dc.description.pubStatus | Published | en_US |
dc.description.oaCategory | CC | en_US |
Appears in Collections: | Journal/Magazine Article |
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File | Description | Size | Format | |
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Chen_Elastin-like_polypeptide.pdf | 17.4 MB | Adobe PDF | View/Open |
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