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Title: Droplet microarray based on nanosensing probe patterns for simultaneous detection of multiple HIV retroviral nucleic acids
Authors: Oudeng, G 
Benz, M
Popova, AA
Zhang, Y
Yi, C
Levkin, PA
Yang, M 
Issue Date: 2020
Source: ACS applied materials and interfaces, 2020, v. 12, no. 50, p. 55614-55623
Abstract: Multiplexed detection of viral nucleic acids is important for rapid screening of viral infection. In this study, we present a molybdenum disulfide (MoS2) nanosheet-modified dendrimer droplet microarray (DMA) for rapid and sensitive detection of retroviral nucleic acids of human immunodeficiency virus-1 (HIV-1) and human immunodeficiency virus-2 (HIV-2) simultaneously. The DMA platform was fabricated by omniphobic–omniphilic patterning on a surface-grafted dendrimer substrate. Functionalized MoS2 nanosheets modified with fluorescent dye-labeled oligomer probes were prepatterned on positively charged amino-modified omniphilic spots to form a fluorescence resonance energy transfer (FRET) sensing microarray. With the formation of separated microdroplets of sample on the hydrophobic–hydrophilic micropattern, prepatterned oligomer probes specifically hybridized with the target HIV genes and detached from the MoS2 nanosheet surface due to weakening of the adsorption force, leading to fluorescence signal recovery. As a proof of concept, we used this microarray with a small sample size (<150 nL) for simultaneous detection of HIV-1 and HIV-2 nucleic acids with a limit of detection (LOD) of 50 pM. The multiplex detection capability was further demonstrated for simultaneous detection of five viral genes (HIV-1, HIV-2, ORFlab, and N genes of SARS-COV-2 and M gene of Influenza A). This work demonstrated the potential of this novel MoS2-DMA FRET sensing platform for high-throughput multiplexed viral nucleic acid screening.
Keywords: Droplet microarray (DMA)
2D MoS2 nanosheets
Fluorescent resonance energy transfer (FRET)
HIV nucleic acids
Publisher: American Chemical Society
Journal: ACS applied materials and interfaces 
ISSN: 1944-8244
EISSN: 1944-8252
DOI: 10.1021/acsami.0c16146
Rights: © 2020 American Chemical Society
This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials & Interfaces, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see
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