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Title: Dynamic simulation of continuous mixed sugar fermentation with increasing cell retention time for lactic acid production using Enterococcus mundtiiQU 25
Authors: Wang, Y 
Chan, KL 
Abdel-Rahman, MA
Sonomoto, K
Leu, SY 
Issue Date: 2020
Source: Biotechnology for biofuels, 2020, v. 13, 112, p. 1-16
Abstract: Background The simultaneous and effective conversion of both pentose and hexose in fermentation is a critical and challenging task toward the lignocellulosic economy. This study aims to investigate the feasibility of an innovative co-fermentation process featuring with a cell recycling unit (CF/CR) for mixed sugar utilization. Al-lactic acid-producing strainEnterococcus mundtiiQU 25 was applied in the continuous fermentation process, and the mixed sugars were utilized at different productivities after the flowing conditions were changed. A mathematical model was constructed with the experiments to optimize the biological process and clarify the cell metabolism through kinetics analysis. The structured model, kinetic parameters, and achievement of the fermentation strategy shall provide new insights toward whole sugar fermentation via real-time monitoring for process control and optimization.
Results Significant carbon catabolite repression in co-fermentation using a glucose/xylose mixture was overcome by replacing glucose with cellobiose, and the ratio of consumed pentose to consumed hexose increased significantly from 0.096 to 0.461 by mass. An outstanding product concentration of 65.2 g L(-1)and productivity of 13.03 g L-1 h(-1)were achieved with 50 g L(-1)cellobiose and 30 g L(-1)xylose at an optimized dilution rate of 0.2 h(-1), and the cell retention time gradually increased. Among the total lactic acid production, xylose contributed to more than 34% of the mixed sugars, which was close to the related contents in agricultural residuals. The model successfully simulated the transition of sugar consumption, cell growth, and lactic acid production among the batch, continuous process, and CF/CR systems.
Conclusion Cell retention time played a critical role in balancing pentose and hexose consumption, cell decay, and lactic acid production in the CF/CR process. With increasing cell concentration, consumption of mixed sugars increased with the productivity of the final product; hence, the impact of substrate inhibition was reduced. With the validated parameters, the model showed the highest accuracy simulating the CF/CR process, and significantly longer cell retention times compared to hydraulic retention time were tested.
Keywords: Lactic acid fermentation
Dilution rate
Cell recycle
Publisher: BioMed Central
Journal: Biotechnology for biofuels 
EISSN: 1754-6834
DOI: 10.1186/s13068-020-01752-6
Description: Correction to : Dynamic simulation of continuous mixed sugar fermentation with increasing cell retention time for lactic acid production using Enterococcus mundtii QU 25 (vol 13, 112, 2020)
Rights: © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.
The following publication Wang, Y., Chan, K. L., Abdel-Rahman, M. A., Sonomoto, K., & Leu, S. Y. (2020). Dynamic simulation of continuous mixed sugar fermentation with increasing cell retention time for lactic acid production usingEnterococcus mundtiiQU 25. Biotechnology For Biofuels, 13, 1-16 is available at
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