Please use this identifier to cite or link to this item:
PIRA download icon_1.1View/Download Full Text
Title: Thermostable beta-lactamase mutant with its active site conjugated with fluorescein for efficient beta-lactam antibiotic detection
Authors: Au, HW 
Tsang, MW 
So, PK 
Wong, KY 
Leung, YC 
Issue Date: 2019
Source: ACS omega, 27 Nov. 2019, v. 4, no. 24, p. 20493-20502
Abstract: Monitoring the beta-lactam antibiotic level has been an important task in food industry and clinical practice. Here, we report the development of a fluorescent PenP beta-lactamase, PenP-E166Cf/N170Q for efficient beta-lactam antibiotic detection. It was constructed by covalently attaching fluorescein onto the active-site entrance of a thermostable E166Cf/N170Q mutant of a Bacillus licheniformis PenP beta-lactamase. It gave a fluorescence turn-on signal toward various beta-lactam antibiotics, where the fluorescence enhancement was attributed to the acyl-enzyme complex formed between PenP-E166Cf/N170Q and the beta-lactam antibiotic. It demonstrated enhanced signal stability over its parental PenP-E166Cf because of the suppressed hydrolytic activity by the N170Q mutation. Compared with our previously constructed PenPC-E166Cf biosensor, PenP-E166Cf/N170Q was more thermostable and advanced in detecting beta-lactams in terms of response time, signal stability, and detection limit. Positive fluorescence signals generated by E166Cf/N170Q in response to the penicillin-containing milk and mouse serum illustrated the feasibility of the biosensor for antibiotic detection in real samples. Taken together, our findings suggest the potential application of PenP-E166Cf/N170Q in biosensing beta-lactam antibiotics.
Publisher: American Chemical Society
Journal: ACS omega 
ISSN: 2470-1343
DOI: 10.1021/acsomega.9b02211
Rights: Copyright © 2019 American Chemical Society
This is an open access article published under an ACS AuthorChoice License (, which permits copying and redistribution of the article or any adaptations for non-commercial purpose
The following publication Au, H. W., Tsang, M. W., So, P. K., Wong, K. Y., & Leung, Y. C. (2019). Thermostable beta-lactamase mutant with its active site conjugated with fluorescein for efficient beta-lactam antibiotic detection. ACS Omega, 4(24), 20493-20502 is available at
Appears in Collections:Journal/Magazine Article

Files in This Item:
File Description SizeFormat 
Au_Thermostable_Beta-Lactamase_Mutant.pdf2.09 MBAdobe PDFView/Open
Open Access Information
Status open access
File Version Version of Record
View full-text via PolyU eLinks SFX Query
Show full item record

Page views

Last Week
Last month
Citations as of Oct 1, 2023


Citations as of Oct 1, 2023


Citations as of Sep 28, 2023


Citations as of Sep 28, 2023

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.