Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/81079
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dc.contributorDepartment of Health Technology and Informatics-
dc.creatorLeung, PHM-
dc.creatorSubramanya, R-
dc.creatorMou, QQ-
dc.creatorLee, KTW-
dc.creatorIslam, F-
dc.creatorGopalan, V-
dc.creatorLu, CT-
dc.creatorLam, AKY-
dc.date.accessioned2019-07-29T03:17:47Z-
dc.date.available2019-07-29T03:17:47Z-
dc.identifier.issn1664-302Xen_US
dc.identifier.urihttp://hdl.handle.net/10397/81079-
dc.language.isoenen_US
dc.publisherFrontiers Research Foundationen_US
dc.rightsCopyright © 2019 Leung, Subramanya, Mou, Lee, Islam, Gopalan, Lu and Lam. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.rightsThe following publication Leung PHM, Subramanya R, Mou Q, Lee KT-W, Islam F, Gopalan V, Lu C-T and Lam AK-Y. (2019) Characterization of Mucosa-Associated Microbiota in Matched Cancer and Non-neoplastic Mucosa From Patients With Colorectal Cancer. Front. Microbiol. 10:1317, 11 pages is available at https://dx.doi.org/10.3389/fmicb.2019.01317en_US
dc.subjectColorectal canceren_US
dc.subjectMucosa-associated microbiotaen_US
dc.subjectDysbiosisen_US
dc.subjectFusobacteriumen_US
dc.subjectBrevundimonasen_US
dc.subject16S rRNA amplicon sequencingen_US
dc.titleCharacterization of mucosa-associated microbiota in matched cancer and non-neoplastic mucosa from patients with colorectal canceren_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage1en_US
dc.identifier.epage11en_US
dc.identifier.volume10en_US
dc.identifier.doi10.3389/fmicb.2019.01317en_US
dcterms.abstractColonic microbiota play important roles in the development of colorectal cancer. We aim to characterise the mucosa-associated microbiota in the tumour as well as the matched non-neoplastic mucosa from patients with colorectal cancer. Microbiota profiling in these samples was done using high-throughput 16S rRNA amplicon sequencing. Our results showed that the microbiota richness and diversity were similar between the tumour and non-neoplastic mucosae. Linear discriminant analysis effect size (LEfSe) analysis identified Fusobacterium and Campylobacter as the key genera of the tumour while Brevundimonas as the key genus of the non-neoplastic mucosa. In patients with shorter survival period, the relative abundance of Fusobacterium and Campylobacter was significantly higher in the tumour. Besides, regardless of the sites, tumour showed higher abundance of Fusobacterium. On the other hand, the relative abundance of Brevundimonas was significantly lower in the tumour. When validated with quantitative ddPCR, we found the absolute numbers of both Fusobacterium and F. nucleatum were significantly higher in the carcinoma from patients with shorter survival period, conventional type of adenocarcinoma in the distal portion of the large intestine (descending colon, sigmoidal colon, and rectum). In conclusion, our study showed a compositional alteration in the mucosa-associated microbiota in the tumour, which may contribute to the progression of colorectal cancer.-
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationFrontiers in microbiology, 12 June 2019, v. 10, 1317, p. 1-11-
dcterms.isPartOfFrontiers in microbiology-
dcterms.issued2019-
dc.identifier.isiWOS:000471159900003-
dc.identifier.scopus2-s2.0-85069223230-
dc.identifier.artn1317en_US
dc.description.validate201907 bcrc-
dc.description.oaVersion of Recorden_US
dc.identifier.FolderNumberOA_Scopus/WOSen_US
dc.description.pubStatusPublisheden_US
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