Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/79840
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dc.contributorDepartment of Health Technology and Informaticsen_US
dc.contributorSchool of Optometryen_US
dc.creatorLi, SKLen_US
dc.creatorShan, SWen_US
dc.creatorLi, HLen_US
dc.creatorCheng, AKWen_US
dc.creatorPan, Fen_US
dc.creatorYip, SPen_US
dc.creatorCivan, MMen_US
dc.creatorTo, CHen_US
dc.creatorDo, CWen_US
dc.date.accessioned2018-12-21T07:13:35Z-
dc.date.available2018-12-21T07:13:35Z-
dc.identifier.issn0146-0404en_US
dc.identifier.urihttp://hdl.handle.net/10397/79840-
dc.language.isoenen_US
dc.publisherAssociation for Research in Vision and Ophthalmologyen_US
dc.rightsCopyright 2018 The Authorsen_US
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/).en_US
dc.rightsThe following publication Li SK-L, Shan S-W, Li H-L, et al. Characterization and regulation of gap junctions in porcine ciliary epithelium. Invest Ophthalmol Vis Sci. 2018;59:3461–3468 is available at https://doi.org/10.1167/iovs.18-24682.en_US
dc.subjectGap junctionsen_US
dc.subjectConnexin 43en_US
dc.subjectCiliary epitheliumen_US
dc.subjectAqueous humor formationen_US
dc.subjectGlaucomaen_US
dc.titleCharacterization and regulation of gap junctions in porcine ciliary epitheliumen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage3461en_US
dc.identifier.epage3468en_US
dc.identifier.volume59en_US
dc.identifier.issue8en_US
dc.identifier.doi10.1167/iovs.18-24682en_US
dcterms.abstractPURPOSE. Gap junctions provide a conduit between the intracellular fluids of the pigmented (PE) and non-pigmented (NPE) ciliary epithelial cells, and are therefore critical in the secretion of the aqueous humor (AH). However, opinions differ concerning the connexin (Cx) composition of the gap junctions. Therefore, we aimed to characterize the expression of Cx in the porcine ciliary epithelium (CE), a favorable model for humans; and determine the contribution of the highest expressed Cx to AH secretion.en_US
dcterms.abstractMethods. Freshly-harvested porcine CE cells were used. The mRNA and protein expressions of gap junctions were assessed by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting (WB), respectively. The relative gene expressions of various Cx were determined by quantitative PCR. The gap junction permeability of isolated PE-NPE cell couplets was evaluated by Lucifer Yellow dye transfer.en_US
dcterms.abstractResults. Using RT-PCR and WB, Cx43, Cx45, Cx47, Cx50, and Cx60 were present in porcine CE, with Cx43 being the most abundant isoform, having over 200-fold higher expression than other Cx. Cx43 was primarily localized in the PE-NPE interface and the basolateral membranes of PE cells. Knockdown of Cx43 by siRNA significantly reduced gene and protein expressions, resulting in reduction of transcellular fluid flow by 90%.en_US
dcterms.abstractConclusions. Cx43 was found to be the major component of gap junctions in porcine CE. Consistent with results from a bovine model, our results support the important role of Cx43 in mediating AH secretion. This finding may shed light on the development of a novel ocular hypotensive agent.en_US
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationInvestigative ophthalmology and visual science, July 2018, v. 59, no. 8, p. 3461-3468en_US
dcterms.isPartOfInvestigative ophthalmology and visual scienceen_US
dcterms.issued2018-
dc.identifier.isiWOS:000438365900014-
dc.identifier.pmid30025101-
dc.identifier.eissn1552-5783en_US
dc.identifier.rosgroupid2017006208-
dc.description.ros2017-2018 > Academic research: refereed > Publication in refereed journalen_US
dc.description.validate201812 bcrcen_US
dc.description.oaVersion of Recorden_US
dc.identifier.FolderNumberOA_IR/PIRA-
dc.description.fundingSourceRGCen_US
dc.description.fundingSourceOthersen_US
dc.description.fundingTextPolyU internal grantsen_US
dc.description.pubStatusPublisheden_US
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