Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/70768
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dc.contributorDepartment of Mechanical Engineering-
dc.creatorBai, GH-
dc.creatorLi, Y-
dc.creatorChu, HK-
dc.creatorWang, KQ-
dc.creatorTan, QL-
dc.creatorXiong, JJ-
dc.creatorSun, D-
dc.date.accessioned2017-12-28T06:18:05Z-
dc.date.available2017-12-28T06:18:05Z-
dc.identifier.issn1475-925X-
dc.identifier.urihttp://hdl.handle.net/10397/70768-
dc.language.isoenen_US
dc.publisherBioMed Centralen_US
dc.rights© The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdo-main/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en_US
dc.rightsThe following publication Bai, G. H., Li, Y., Chu, H. K., Wang, K. Q., Tan, Q. L., Xiong, J. J., & Sun, D. (2017). Characterization of biomechanical properties of cells through dielectrophoresis-based cell stretching and actin cytoskeleton modeling. Biomedical Engineering Online, 16, 41, 1-15 is available at https://dx.doi.org/10.1186/s12938-017-0329-8en_US
dc.subjectDielectrophoresisen_US
dc.subjectCell stretchingen_US
dc.subjectCytoskeleton modelen_US
dc.subjectOptical tweezersen_US
dc.subjectMechanical propertyen_US
dc.titleCharacterization of biomechanical properties of cells through dielectrophoresis-based cell stretching and actin cytoskeleton modelingen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.epage15-
dc.identifier.volume16-
dc.identifier.doi10.1186/s12938-017-0329-8-
dcterms.abstractBackground: Cytoskeleton is a highly dynamic network that helps to maintain the rigidity of a cell, and the mechanical properties of a cell are closely related to many cellular functions. This paper presents a new method to probe and characterize cell mechanical properties through dielectrophoresis (DEP)-based cell stretching manipulation and actin cytoskeleton modeling. Methods: Leukemia NB4 cells were used as cell line, and changes in their biological properties were examined after chemotherapy treatment with doxorubicin (DOX). DEPintegrated microfluidic chip was utilized as a low-cost and efficient tool to study the deformability of cells. DEP forces used in cell stretching were first evaluated through computer simulation, and the results were compared with modeling equations and with the results of optical stretching (OT) experiments. Structural parameters were then extracted by fitting the experimental data into the actin cytoskeleton model, and the underlying mechanical properties of the cells were subsequently characterized. Results: The DEP forces generated under different voltage inputs were calculated and the results from different approaches demonstrate good approximations to the force estimation. Both DEP and OT stretching experiments confirmed that DOX-treated NB4 cells were stiffer than the untreated cells. The structural parameters extracted from the model and the confocal images indicated significant change in actin network after DOX treatment. Conclusion: The proposed DEP method combined with actin cytoskeleton modeling is a simple engineering tool to characterize the mechanical properties of cells.-
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationBioMedical engineering online, 2017, v. 16, 41, p. 1-15-
dcterms.isPartOfBioMedical engineering online-
dcterms.issued2017-
dc.identifier.isiWOS:000398632600001-
dc.identifier.pmid28376803-
dc.identifier.ros2016000934-
dc.identifier.artn41-
dc.identifier.rosgroupid2016000919-
dc.description.ros2016-2017 > Academic research: refereed > Publication in refereed journal-
dc.description.validatebcrc-
dc.description.oaVersion of Recorden_US
dc.identifier.FolderNumberOA_IR/PIRAen_US
dc.description.pubStatusPublisheden_US
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