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| Title: | A fluorescence turn-on biosensor based on graphene quantum dots (GQDs) and molybdenum disulfide (MoS2) nanosheets for epithelial cell adhesion molecule (EpCAM) detection | Authors: | Shi, JY Lyu, J Tian, F Yang, M |
Issue Date: | 15-Jul-2017 | Source: | Biosensors and bioelectronics, 15 July 2017, v. 93, p. 182-188 | Abstract: | This paper presents a "turn-on" fluorescence biosensor based on graphene quantum dots (GQDs) and molybdenum disulfide (MoS2) nanosheets for rapid and sensitive detection of epithelial cell adhesion molecule (EpCAM). PEGylated GQDs were used as donor molecules, which could not only largely increase emission intensity but also prevent non-specific adsorption of PEGylated GQD on MoS2 surface. The sensing platform was realized by adsorption of PEGylated GQD labelled EpCAM aptamer onto MoS2 surface via van der Waals force. The fluorescence signal of GQD was then quenched by MoS2 nanosheets via fluorescence resonance energy transfer (FRET) mechanism. In the presence of EpCAM protein, the stronger specific affinity interaction between aptamer and EpCAM protein could detach GQD labelled EpCAM aptamer from MoS2 nanosheets, leading to the restoration of fluorescence intensity. By monitoring the change of fluorescence signal, the target EpCAM protein could be detected sensitively and selectively with a linear detection range from 3 nM to 54 nM and limit of detection (LOD) around 450 pM. In addition, this nanobiosensor has been successfully used for EpCAM-expressed breast cancer MCF-7 cell detection. | Keywords: | Graphene quantum dots (GQDs) Molybdenum disulfide (MoS2) Fluorescence resonance energy transfer (FRET) Epithelial cell adhesion molecule (EpCAM) |
Publisher: | Elsevier | Journal: | Biosensors and bioelectronics | ISSN: | 0956-5663 | EISSN: | 1873-4235 | DOI: | 10.1016/j.bios.2016.09.012 | Rights: | © 2016 Elsevier B.V. All rights reserved. © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ The following publication Shi, J., Lyu, J., Tian, F., & Yang, M. (2017). A fluorescence turn-on biosensor based on graphene quantum dots (GQDs) and molybdenum disulfide (MoS2) nanosheets for epithelial cell adhesion molecule (EpCAM) detection. Biosensors and Bioelectronics, 93, 182-188 is available at https://doi.org/10.1016/j.bios.2016.09.012 |
| Appears in Collections: | Journal/Magazine Article |
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